Please use this identifier to cite or link to this item:
https://hdl.handle.net/11147/4904
Title: | Imatinib-induced apoptosis: A possible link to topoisomerase enzyme inhibition | Authors: | Baran, Yusuf Zencir, Sevil Çakır, Zeynep Öztürk, Esra Topçu, Zeki |
Keywords: | BCR/ABL Apoptosis Topoisomerase Chronic myeloid leukaemia Imatinib |
Publisher: | John Wiley and Sons Inc. | Source: | Baran, Y., Zencir, S., Çakır, Z., Öztürk, E., and Topçu, Z. (2011). Imatinib-induced apoptosis: A possible link to topoisomerase enzyme inhibition. Journal of Clinical Pharmacy and Therapeutics, 36(6), 673-679. doi:10.1111/j.1365-2710.2010.01224.x | Abstract: | Summary What is known and Objective: Imatinib is a specific BCR/ABL inhibitor, commonly used for the treatment of chronic myeloid leukaemia (CML), a hematological malignancy resulting from a chromosomal translocation that generates the BCR/ABL fusion protein. Recent studies showed that the imatinib has cytotoxic and apoptotic effects on many BCR/ABL-negative cancers. Numerous compounds with cytotoxic potential exert their functions by interfering with the DNA topoisomerase. In this study, we examined the effects of imatinib on tumour cell-killing in relation to DNA topoisomerase enzyme inhibition. Methods: We determined the cytotoxicity by cell proliferation assay (XTT; tetrazolium hydroxide), using the human K562 CML cells, and loss of mitochondrial membrane potential by monitoring the changes in caspase-3 enzyme activity. Type I and II topoisomerase activities were measured by supercoiled plasmid relaxation and minicircle DNA decatenation assays respectively. Results and Discussion: Imatinib-induced apoptosis and inhibited cell proliferation in a dose-dependent manner. We also found that the imatinib was effective in both type I and type II topoisomerase reactions to a varying degree between 94% and 7% for the concentration range of 1 mm-0.02 mm in a dose-dependent manner. What is new and Conclusion: Our results suggest that the inhibition of topoisomerases may be a significant factor in imatinib-induced apoptosis in CML. | URI: | http://doi.org/10.1111/j.1365-2710.2010.01224.x http://hdl.handle.net/11147/4904 |
ISSN: | 0269-4727 0269-4727 |
Appears in Collections: | Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
Show full item record
CORE Recommender
SCOPUSTM
Citations
7
checked on Sep 1, 2024
WEB OF SCIENCETM
Citations
5
checked on Aug 24, 2024
Page view(s)
222
checked on Sep 2, 2024
Download(s)
214
checked on Sep 2, 2024
Google ScholarTM
Check
Altmetric
Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.