Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5083
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dc.contributor.authorGunasekaran, Karthikeyan-
dc.contributor.authorNguyen, Thi H.-
dc.contributor.authorMaynard, Heather D.-
dc.contributor.authorDavis, Thomas P.-
dc.contributor.authorBulmuş, Volga-
dc.date.accessioned2017-03-17T11:13:23Z
dc.date.available2017-03-17T11:13:23Z
dc.date.issued2011-04
dc.identifier.citationGunasekaran, K., Nguyen, T.H., Maynard, H.D., Davis, T.P., and Bulmuş, V. (2001). Conjugation of siRNA with comb-type PEG enhances serum stability and gene silencing efficiency. Macromolecular Rapid Communications, 32(8), 654-659. doi:10.1002/marc.201000804en_US
dc.identifier.issn1022-1336
dc.identifier.issn1022-1336-
dc.identifier.issn1521-3927-
dc.identifier.urihttps://doi.org/10.1002/marc.201000804
dc.identifier.urihttp://hdl.handle.net/11147/5083
dc.description.abstractA thiol-modified siRNA targeting the enhanced green fluorescence protein (eGFP) gene was conjugated with RAFT-synthesized, pyridyl disulfide-functional poly(PEG methyl ether acrylate)s (p(PEGA)s). siRNA-p(PEGA) conjugates demonstrated significantly enhanced in vitro serum stability and nuclease resistance compared to the unmodified and thiol-modified siRNA. The complexes of siRNA-p(PEGA) conjugates with a fusogenic peptide, KALA ((+)/(-) = 2) inhibited the protein expression approximately 28-fold more than the KALA complex of the unmodified siRNA. The protein inhibition caused by siRNA-p(PEGA)-KALA complexes (56± 5%-58± 3% of the fluorescence expressed in non-treated cells) was comparable to the effect of the unmodified siRNA-lipofectamine complex (77± 7%).en_US
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Inc.en_US
dc.relation.ispartofMacromolecular Rapid Communicationsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPEG(meth)acrylateen_US
dc.subjectsiRNAen_US
dc.subjectBiological applications of polymersen_US
dc.subjectPolymer conjugatesen_US
dc.subjectRAFT polymerizationen_US
dc.subjectReversible addition fragmentation chain transfer polymerizationen_US
dc.titleConjugation of siRNA with comb-type PEG enhances serum stability and gene silencing efficiencyen_US
dc.typeArticleen_US
dc.authoridTR181383en_US
dc.institutionauthorBulmuş, Volga-
dc.departmentİzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume32en_US
dc.identifier.issue8en_US
dc.identifier.startpage654en_US
dc.identifier.endpage659en_US
dc.identifier.wosWOS:000289376200005en_US
dc.identifier.scopus2-s2.0-79953844934en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1002/marc.201000804-
dc.identifier.pmid21480423en_US
dc.relation.doi10.1002/marc.201000804en_US
dc.coverage.doi10.1002/marc.201000804en_US
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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