Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5448
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dc.contributor.authorHorzum, Utku-
dc.contributor.authorÖzdil, Berrin-
dc.contributor.authorPesen Okvur, Devrim-
dc.date.accessioned2017-05-05T07:14:58Z-
dc.date.available2017-05-05T07:14:58Z-
dc.date.issued2014-06-
dc.identifier.citationHorzum, U., Özdil, B., and Pesen Okvur, D. (2014). Micrometer scale spacings between fibronectin nanodots regulate cell morphology and focal adhesions. Materials Research Express, 1(2). doi:10.1088/2053-1591/1/2/025402en_US
dc.identifier.issn2053-1591-
dc.identifier.urihttps://doi.org/10.1088/2053-1591/1/2/025402-
dc.identifier.urihttp://hdl.handle.net/11147/5448-
dc.description.abstractCell adhesion to extracellular matrix is an important process for both health and disease states. Surface protein patterns that are topographically flat, and do not introduce other chemical, topographical or rigidity related functionality and, more importantly, that mimic the organization of the in vivo extracellular matrix are desired. Previous work showed that vinculin and cytoskeletal organization are modulated by size and shape of surface nanopatterns. However, quantitative analysis on cell morphology and focal adhesions as a function of micrometer scale spacings of FN nanopatterns was absent. Here, electron beam lithography was used to pattern fibronectin nanodots with micrometer scale spacings on a Kcasein background on indium tin oxide coated glass which, unlike silicon, is transparent and thus suitable for many light microscopy techniques. Exposure times were significantly reduced using the line exposure mode with micrometer scale step sizes. Micrometer scale spacings of 2, 4 and 8 m between fibronectin nanodots proved to modulate cell adhesion through modification of cell area, focal adhesion number, size and circularity. Overall, cell behavior was shown to shift at the apparent threshold of 4 m spacing. The findings presented here offer exciting new opportunities for cell biology research.en_US
dc.description.sponsorshipTUBITAK (111T026); DPT (2009K120860)en_US
dc.language.isoenen_US
dc.publisherIOP Publishing Ltd.en_US
dc.relation.ispartofMaterials Research Expressen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCell shapeen_US
dc.subjectElectron beam lithographyen_US
dc.subjectFibronectinen_US
dc.subjectFocal adhesionen_US
dc.titleMicrometer scale spacings between fibronectin nanodots regulate cell morphology and focal adhesionsen_US
dc.typeArticleen_US
dc.authoridTR114932en_US
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume1en_US
dc.identifier.issue2en_US
dc.identifier.wosWOS:000209665000051
dc.identifier.scopusSCOPUS:2-s2.0-84945246058
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1088/2053-1591/1/2/025402-
dc.relation.doi10.1088/2053-1591/1/2/025402en_US
dc.coverage.doi10.1088/2053-1591/1/2/025402en_US
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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