Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5566
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dc.contributor.authorSevimli, Sema-
dc.contributor.authorSagnella, Sharon-
dc.contributor.authorMacmillan, Alexander-
dc.contributor.authorWhan, Renee-
dc.contributor.authorKavallaris, Maria-
dc.contributor.authorBulmuş, Volga-
dc.contributor.authorDavis, Thomas P.-
dc.date.accessioned2017-05-22T11:07:54Z-
dc.date.available2017-05-22T11:07:54Z-
dc.date.issued2015-02-
dc.identifier.citationSevimli, S., Sagnella, S., Macmillan, A., Whan, R., Kavallaris, M., Bulmuş, V., and Davis, T. P. (2015). The endocytic pathway and therapeutic efficiency of doxorubicin conjugated cholesterol-derived polymers. Biomaterials Science, 3(2), 323-335. doi:10.1039/c4bm00224een_US
dc.identifier.issn2047-4830-
dc.identifier.urihttp://doi.org/10.1039/c4bm00224e-
dc.identifier.urihttp://hdl.handle.net/11147/5566-
dc.description.abstractPreviously synthesized poly(methacrylic acid-co-cholesteryl methacrylate) P(MAA-co-CMA) copolymers were examined as potential drug delivery vehicles. P(MAA-co-CMA) copolymers were fluorescently labelled and imaged in SHEP and HepG2 cells. To understand their cell internalization pathway endocytic inhibition studies were conducted. It was concluded that P(MAA-co-CMA) are taken up by the cells via clathrin-independent endocytosis (CIE) (both caveolae mediated and cholesterol dependent endocytosis) mechanisms. The formation and characterization of P(MAA-co-CMA)-doxorubicin (DOX) nanocomplexes was investigated by fluorescence lifetime imaging microscopy (FLIM), UV-Visible spectroscopy (UV-Vis) and dynamic light scattering (DLS) studies. The toxicity screening between P(MAA-co-CMA)-DOX nanocomplexes (at varying w/w ratios) and free DOX, revealed nanocomplexes to exhibit higher cytotoxicity towards cancer cells in comparison to normal cells. FLIM and confocal microscopy were employed for investigating the time-dependent release of DOX in SHEP cells and the cellular uptake profile of P(MAA-co-CMA)-DOX nanocomplexes in cancer and normal cell lines, respectively. The endocytic pathway of P(MAA-co-CMA)-DOX nanocomplexes were examined in SHEP and HepG2 cells via flow cytometry revealing the complexes to be internalized through both clathrin-dependent (CDE) and CIE mechanisms. The drug delivery profile, reported herein, illuminates the specific endocytic route and therapeutic efficiency of P(MAA-co-CMA)-DOX nanocomplexes strongly suggesting these particles to be promising candidates for in vivo applications.en_US
dc.description.sponsorshipNHMRC Senior Research Fellowship (APP1058299)en_US
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofBiomaterials Scienceen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCellsen_US
dc.subjectCholesterolen_US
dc.subjectConjugated polymersen_US
dc.subjectTherapeutic efficiencyen_US
dc.subjectPolyacrylatesen_US
dc.subjectEndocytic pathwaysen_US
dc.titleThe endocytic pathway and therapeutic efficiency of doxorubicin conjugated cholesterol-derived polymersen_US
dc.typeArticleen_US
dc.authoridTR181383en_US
dc.institutionauthorBulmuş, Volga-
dc.departmentİzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume3en_US
dc.identifier.issue2en_US
dc.identifier.startpage323en_US
dc.identifier.endpage335en_US
dc.identifier.wosWOS:000348202600012en_US
dc.identifier.scopus2-s2.0-84921626524en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1039/c4bm00224e-
dc.identifier.pmid26218123en_US
dc.relation.doi10.1039/c4bm00224een_US
dc.coverage.doi10.1039/c4bm00224een_US
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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