Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5590
Full metadata record
DC FieldValueLanguage
dc.contributor.authorÖzdil, Berrin-
dc.contributor.authorÖnal, Sevgi-
dc.contributor.authorOruç, Tuğçe-
dc.contributor.authorPesen Okvur, Devrim-
dc.date.accessioned2017-05-24T06:58:53Z
dc.date.available2017-05-24T06:58:53Z
dc.date.issued2014
dc.identifier.citationÖzdil, B., Önal, S., Oruç, T., and Pesen Okvur, D. (2014). Fabrication of 3D Controlled in vitro Microenvironments. MethodsX, 1(1), 60-66. doi:10.1016/j.mex.2014.06.003en_US
dc.identifier.issn2215-0161
dc.identifier.issn2215-0161-
dc.identifier.urihttps://doi.org/10.1016/j.mex.2014.06.003
dc.identifier.urihttp://hdl.handle.net/11147/5590
dc.description.abstractMicrofluidics-based lab-on-a-chips have many advantages, one of which is to provide physiologically relevant settings for cell biology experiments. Thus there is an ever increasing interest in their fabrication. Our goal is to construct three dimensional (3D) Controlled in vitro Microenvironments (CivMs) that mimic the in vivo microenvironments. Here, we present our optimized fabrication method that works for various lab-on-a-chip designs with a wide range of dimensions. The most crucial points are:While using one type of SU-8 photoresist (SU-2075), fine tuning of ramp, dwell time, spin speed, durations of soft bake, UV exposure and development allows fabrication of SU-8 masters with various heights from 40 to 600 μm.Molding PDMS (polydimethylsiloxane) at room temperature for at least two days instead of baking at higher temperatures prevents not only tears and bubbles in PDMS stamps but also cracks in the SU-8 master.3D nature of the CivMs is ensured by keeping the devices inverted during gel polymerization.en_US
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.relation.ispartofMethodsXen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHydrogelen_US
dc.subjectLab-on-a-chipen_US
dc.subjectMatrigelen_US
dc.subjectMicroenvironmenten_US
dc.subjectMicrofluidic deviceen_US
dc.subjectPolydimethylsiloxaneen_US
dc.titleFabrication of 3D controlled in vitro microenvironmentsen_US
dc.typeArticleen_US
dc.authoridTR114932en_US
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume1en_US
dc.identifier.issue1en_US
dc.identifier.startpage60en_US
dc.identifier.endpage66en_US
dc.identifier.scopusSCOPUS:2-s2.0-84905160411
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.mex.2014.06.003-
dc.relation.doi10.1016/j.mex.2014.06.003en_US
dc.coverage.doi10.1016/j.mex.2014.06.003en_US
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Files in This Item:
File Description SizeFormat 
5590.pdfMakale330.65 kBAdobe PDFThumbnail
View/Open
Show simple item record

CORE Recommender

SCOPUSTM   
Citations

5
checked on Oct 23, 2021

Page view(s)

38
checked on Oct 26, 2021

Download(s)

30
checked on Oct 26, 2021

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.