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https://hdl.handle.net/11147/5647
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DC Field | Value | Language |
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dc.contributor.author | Atik, Ahmet Emin | - |
dc.contributor.author | Hernandez, Oscar | - |
dc.contributor.author | Maitre, Philippe | - |
dc.contributor.author | Yalçın, Talat | - |
dc.date.accessioned | 2017-05-30T13:10:13Z | - |
dc.date.available | 2017-05-30T13:10:13Z | - |
dc.date.issued | 2014-12 | - |
dc.identifier.citation | Atik, A.E., Hernandez, O., Maître, P., and Yalçın, T. (2014). Specific rearrangement reactions of acetylated lysine containing peptide bn (n=4-7) ion series. Journal of Mass Spectrometry, 49(12), 1290-1297. doi:10.1002/jms.3462 | en_US |
dc.identifier.issn | 1076-5174 | - |
dc.identifier.uri | https://doi.org/10.1002/jms.3462 | - |
dc.identifier.uri | http://hdl.handle.net/11147/5647 | - |
dc.description.abstract | Characterization of ε-N-acetylated lysine containing peptides, one of the most prominent post-translational modifications of proteins, is an important goal for tandem mass spectrometry experiments. A systematic study for the fragmentation reactions of b ions derived from ε-N-acetyllysine containing model octapeptides (KAcYAGFLVG and YAKAcGFLVG) has been examined in detail. Collision-induced dissociation (CID) mass spectra of bn (n=4-7) fragments of ε-N-acetylated lysine containing peptides are compared with those of N-terminal acetylated and doubly acetylated (both ε-N and N-terminal) peptides, as well as acetyl-free peptides. Both direct and nondirect fragments are observed for acetyl-free and singly acetylated (ε-N or N-terminal) peptides. In the case of ε-N-acetylated lysine containing peptides, however, specific fragment ions (m/z 309, 456, 569 and 668) are observed in CID mass spectra of bn (n=4-7) ions. The CID mass spectra of these four ions are shown to be identical to those of selected protonated C-terminal amidated peptides. On this basis, a new type of rearrangement chemistry is proposed to account for the formation of these fragment ions,which are specific for ε-N-acetylated lysine containing peptides. Consistent with the observation of nondirect fragments, it is proposed that the b ions undergo head-to-tail macrocyclization followed by ring opening. The proposed reaction pathway assumes that bn (n=4-7) of ε-N-acetylated lysine containing peptides has a tendency to place the KAc residue at the C-terminal position after macrocyclization/reopening mechanism. Then, following the loss of CO, it is proposed that the marker ions are the result of the loss of an acetyllysine imine as a neutral fragment. | en_US |
dc.description.sponsorship | Scientific and Technological Research Council of Turkey (113Z172); State Planning Organization, DPT; Reseau National de Spectrometrie de Masse FT - ICR a tres haut champ (FR3624) | en_US |
dc.language.iso | en | en_US |
dc.publisher | John Wiley and Sons Inc. | en_US |
dc.relation | info:eu-repo/grantAgreement/TUBITAK/KBAG/113Z172 | en_US |
dc.relation.ispartof | Journal of Mass Spectrometry | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Macrocyclization of b ions | en_US |
dc.subject | Peptide fragmentation | en_US |
dc.subject | Lysine acetylation | en_US |
dc.subject | Peptides | en_US |
dc.title | Specific Rearrangement Reactions of Acetylated Lysine Containing Peptide Bn (n=4-7) Ion Series | en_US |
dc.type | Article | en_US |
dc.authorid | TR130617 | en_US |
dc.institutionauthor | Atik, Ahmet Emin | - |
dc.institutionauthor | Yalçın, Talat | - |
dc.department | İzmir Institute of Technology. Chemistry | en_US |
dc.identifier.volume | 49 | en_US |
dc.identifier.issue | 12 | en_US |
dc.identifier.startpage | 1290 | en_US |
dc.identifier.endpage | 1297 | en_US |
dc.identifier.wos | WOS:000345998800012 | en_US |
dc.identifier.scopus | 2-s2.0-84916207773 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1002/jms.3462 | - |
dc.identifier.pmid | 25476947 | en_US |
dc.relation.doi | 10.1002/jms.3462 | en_US |
dc.coverage.doi | 10.1002/jms.3462 | en_US |
dc.identifier.wosquality | Q2 | - |
dc.identifier.scopusquality | Q2 | - |
item.fulltext | With Fulltext | - |
item.openairetype | Article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
crisitem.author.dept | 04.01. Department of Chemistry | - |
Appears in Collections: | Chemistry / Kimya PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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