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https://hdl.handle.net/11147/5669
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Palaniappan, Al. | - |
dc.contributor.author | Cheema, Jamal Ahmed | - |
dc.contributor.author | Rajwar, Deepa | - |
dc.contributor.author | Ammanath, Gopal | - |
dc.contributor.author | Xiaohu, Liu | - |
dc.contributor.author | Seng Koon, Lim | - |
dc.contributor.author | Yi, Wang | - |
dc.contributor.author | Yıldız, Ümit Hakan | - |
dc.contributor.author | Liedberg, Bo | - |
dc.date.accessioned | 2017-06-01T08:34:26Z | - |
dc.date.available | 2017-06-01T08:34:26Z | - |
dc.date.issued | 2015-12 | - |
dc.identifier.citation | Palaniappan, A., Cheema, J. A., Rajwar, D., Ammanath, G., Xiaohu, L., Seng Koon, L., Yi, W., Yıldız, Ü. H., and Liedberg, B. (2015). Polythiophene derivative on quartz resonators for miRNA capture and assay, Analyst, 140(23), 7912-7917. doi:10.1039/c5an01663k | en_US |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.issn | 1364-5528 | - |
dc.identifier.uri | http://doi.org/10.1039/c5an01663k | - |
dc.identifier.uri | http://hdl.handle.net/11147/5669 | - |
dc.description.abstract | A novel approach for miRNA assay using a cationic polythiophene derivative, poly[3-(3′-N,N,N-triethylamino-1′-propyloxy)-4-methyl-2,5-thiophene hydrobromide] (PT), immobilized on a quartz resonator is proposed. The cationic PT enables capturing of all RNA sequences in the sample matrix via electrostatic interactions, resulting in the formation of PT-RNA duplex structures on quartz resonators. Biotinylated peptide nucleic acid (b-PNA) sequences are subsequently utilized for the RNA assay, upon monitoring the PT-RNA-b-PNA triplex formation. Signal amplification is achieved by anchoring avidin coated nanoparticles to b-PNA in order to yield responses at clinically relevant concentration regimes. Unlike conventional nucleic acid assay methodologies that usually quantify a specific sequence of RNA, the proposed approach enables the assay of any RNA sequence in the sample matrix upon hybridization with a PNA sequence complementary to the RNA of interest. As an illustration, successful detection of mir21, (a miRNA sequence associated with lung cancer) is demonstrated with a limit of detection of 400 pM. Furthermore, precise quantification of mir21 in plasma samples is demonstrated without requiring PCR and sophisticated instrumentation. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Royal Society of Chemistry | en_US |
dc.relation.ispartof | Analyst | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | MicroRNAs | en_US |
dc.subject | Polymers | en_US |
dc.subject | Polythiophene | en_US |
dc.subject | Thiophenes | en_US |
dc.subject | Quartz crystal | en_US |
dc.title | Polythiophene derivative on quartz resonators for miRNA capture and assay | en_US |
dc.type | Article | en_US |
dc.authorid | TR147447 | en_US |
dc.institutionauthor | Yıldız, Ümit Hakan | - |
dc.department | İzmir Institute of Technology. Chemistry | en_US |
dc.identifier.volume | 140 | en_US |
dc.identifier.issue | 23 | en_US |
dc.identifier.startpage | 7912 | en_US |
dc.identifier.endpage | 7917 | en_US |
dc.identifier.wos | WOS:000365189900010 | en_US |
dc.identifier.scopus | 2-s2.0-84947080668 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1039/c5an01663k | - |
dc.identifier.pmid | 26478920 | en_US |
dc.relation.doi | 10.1039/c5an01663k | en_US |
dc.coverage.doi | 10.1039/c5an01663k | en_US |
dc.identifier.wosquality | Q2 | - |
dc.identifier.scopusquality | Q2 | - |
item.languageiso639-1 | en | - |
item.openairetype | Article | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | 04.01. Department of Chemistry | - |
Appears in Collections: | Chemistry / Kimya PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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