Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5787
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dc.contributor.authorMoghtader, Farzaneh-
dc.contributor.authorÇongur, Gülşah-
dc.contributor.authorZareie, Hadi M.-
dc.contributor.authorErdem, Arzum-
dc.contributor.authorPişkin, Erhan-
dc.date.accessioned2017-06-16T08:39:40Z
dc.date.available2017-06-16T08:39:40Z
dc.date.issued2016
dc.identifier.citationMoghtader, F., Çongur, G., Zareie, H. M., Erdem, A., and Pişkin, E. (2016). Impedimetric detection of pathogenic bacteria with bacteriophages using gold nanorod deposited graphite electrodes. RSC Advances, 6(100), 97832-97839. doi:10.1039/c6ra18884ben_US
dc.identifier.issn2046-2069
dc.identifier.issn2046-2069-
dc.identifier.urihttp://doi.org/10.1039/c6ra18884b
dc.identifier.urihttp://hdl.handle.net/11147/5787
dc.description.abstractElectrochemical impedance spectroscopy (EIS) is applied for the detection of bacteria using bacteriophages as a bioprobe together with gold nanorods (GNRs). Escherichia coli-E. coli K12 was used as a model target bacteria and also for the propagation of its specific T4-phages. Gold nanorods (GNRs) were synthesized via a two-step protocol and characterized using different techniques. EIS measurements were conducted in an electrochemical cell consisting of a three electrode system. Single-use pencil graphite electrodes (PGE) were modified by the physical adsorption of GNRs to increase their interfacial conductivity and therefore sensitivity for impedimetric measurements. Therefore, interfacial charge-transfer resistance values (Rct) sharply decreased after GNRs deposition. Phages were adsorbed on these electrodes via a simple incubation protocol at room temperature, which resulted in an increase in Rct values, which was concluded to be as a result of nonconductive phage layers. These phage-carrying GNRs-PGEs were used for impedimetric detection of the target bacteria, E. coli. Significant increases at the Rct values were observed which were attributed to the insulation effects of the adsorbed bacterial layers. This increase was even more when the bacterial concentrations were higher. In the case of the non-target bacteria Staphylococcus aureus (S. aureus), conductivity noticeable decreases (due to nonspecific adsorption). However, in the case of E. coli, the Rct value increase is time dependent and reaches maximum in about 25-30 min, then decreases gradually as a result of bacterial lysis due to phage invasion on the electrode surfaces. In contrast, there were no time dependent changes with the non-target bacteria S. aureus (no infection and no lytic activity). It is concluded that the target bacteria could be detected using this very simple and inexpensive detection protocol with a minimum detection limit of 103 CFU mL-1 in approximately 100 μL bacterial suspension.en_US
dc.description.sponsorshipEU-FP7-IAPP Nanobacterphage-SERS; Biyomedtek/NanoBMTen_US
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofRSC Advancesen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGraphite electrodesen_US
dc.subjectGolden_US
dc.subjectBacteriophagesen_US
dc.subjectCharge transferen_US
dc.subjectEscherichia colien_US
dc.subjectNanoribbonsen_US
dc.subjectBacteriophagesen_US
dc.titleImpedimetric detection of pathogenic bacteria with bacteriophages using gold nanorod deposited graphite electrodesen_US
dc.typeArticleen_US
dc.authoridTR166094en_US
dc.institutionauthorZareie, Hadi M.-
dc.departmentİzmir Institute of Technology. Materials Science and Engineeringen_US
dc.identifier.volume6en_US
dc.identifier.issue100en_US
dc.identifier.startpage97832en_US
dc.identifier.endpage97839en_US
dc.identifier.wosWOS:000386312700039en_US
dc.identifier.scopus2-s2.0-84992436581en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1039/c6ra18884b-
dc.relation.doi10.1039/c6ra18884ben_US
dc.coverage.doi10.1039/c6ra18884ben_US
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.openairetypeArticle-
Appears in Collections:Materials Science and Engineering / Malzeme Bilimi ve Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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