Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6146
Title: The aggregatibacter actinomycetemcomitans heat shock protein GroEL interacts directly with human peripheral blood T cells
Authors: Nalbant, Ayten
Saygılı, Tahsin
Nalbant, Ayten
Saygılı, Tahsin
Keywords: T cells
Aggregatibacter actinomycetemcomitans GroEL
Cytokines
Hsp64
TLR2
Issue Date: 2016
Publisher: TUBITAK
Source: Nalbant, A., and Saygılı, T. (2016). The aggregatibacter actinomycetemcomitans heat shock protein GroEL interacts directly with human peripheral blood T cells. Turkish Journal of Biology, 40(6), 1140-1147.
Abstract: Heat shock family protein GroEL of Aggregatibacter actinomycetemcomitans (Aa) has antigenic properties. We previously demonstrated that A. actinomycetemcomitans GroEL-like protein affects human CD4 T cells by converting them into IL-10 and IFNg double cytokine producing Tbet+ Th1 cells. The objective of this study was to investigate whether or not AaGroEL communicates with T cells directly. To do this, sorted cells from peripheral blood mononuclear cells were stimulated with AaGroEL for 48 h. Flow cytometry was used to measure soluble and intracellular cytokine expression in the cell cultures and detect TLR2 expression on the surface of T cells. Expression of six different soluble cytokines was evaluated by CBA assay. To determine whether AaGroEL affects CD3+ T cells directly or not, purified CD3+ T cells or CD14+ cells were cultured with AaGroEL separately, and the quantity of soluble cytokine was measured. Results showed that sorted CD3+ cells produced soluble IL-6, TNFα-and IFNγ cytokines. Additionally, the intracellular cytokine staining data showed that AaGroEL-stimulated CD3+ cells were also TNFα-and IFNγ-positive. Moreover, AaGroEL-responsive T cells slightly increased their TLR2 expression. These findings suggest that CD3+ T cells produce cytokines in response to AaGroEL protein without requirements for other cells, such as CD14+ monocytes.
URI: http://hdl.handle.net/11147/6146
ISSN: 1300-0152
1300-0152
1303-6092
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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