Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6273
Title: Protective effects of polyamines against UV-A and UV-B illumination in Physcia semipinnata thalli
Authors: Esmer, Işıl
Tüney, İnci
Ünal Özakça, Dilek
Sukatar, Atakan
Keywords: Lipid peroxidation
Putrescine
RAPD-PCR
Spermidine
Issue Date: 2017
Publisher: Institute of Botany and Botanical Garden Jevremovac
Source: Esmer, I., Tüney, İ., Ünal Özakça, D., and Sukatar, A. (2017). Protective effects of polyamines against UV-A and UV-B illumination in Physcia semipinnata thalli. Botanica Serbica, 41(1), 17-24. doi:10.5281/zenodo.453042
Abstract: The damage to DNA induced by UV-A and UV-B and protective effects of the polyamines putrescine (put), spermidine (spd) and spermine (spm) were investigated on the lichen Physcia semipinnata in the present study. Our results suggest that significant alterations of the photosynthetic quantum yield ratio occurred in response to increased UV-A and UV-B exposure time. The photosynthetic quantum yield ratio gradually decreased in P. semipinnata following exposure to UV-A and UV-B. Physcia semipinnata thalli which were treated with a polyamine in a concentration of 1 mM were not affected by UV-A exposure for 72 h. In the case of UV-B treatment, the protective polyamine dosage was 0.25 mM. We also used the random amplified polymorphic DNA (RAPD) technique to detect DNA damage. The main changes observed in the RAPD profiles, which were obtained using 12 RAPD primers, were the appearance or disappearance of different bands and variation of their intensities. The use of at least three different primers allowed detection of specific band patterns in both UV-A- and UV-B-exposed samples treated with polyamines as compared to untreated ones.
URI: http://doi.org/10.5281/zenodo.453042
http://hdl.handle.net/11147/6273
ISSN: 1821-2158
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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