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Title: Polyglycolide-montmorillonite as a novel nanocomposite platform for biosensing applications
Authors: Ünal, Betül
Yalçınkaya, Esra Evrim
Gümüştaş, Sıla
Sönmez, Burak
Özkan, Melek
Balcan, Mehmet
Odacı Demirkol, Dilek
Timur, Suna
Keywords: Nanocomposites
Pyranose oxidase
Issue Date: 2017
Publisher: Royal Society of Chemistry
Source: Ünal, B., Yalçınkaya, E.E., Gümüştaş, S., Sönmez, B., Özkan, M., Balcan, M., Odacı Demirkol, D., and Timur, S. (2017). Polyglycolide-montmorillonite as a novel nanocomposite platform for biosensing applications. New Journal of Chemistry, 41(17), 9371-9379. doi:10.1039/c7nj01751k
Abstract: In catalytic biosensors, the immobilization of biomolecules in a suitable matrix is one of the vital parameters for obtaining improved systems. Clays, which are intercalated with various organic compounds, have a great tendency to develop biosensors with high stability, sensitivity and reproducibility. Herein, a polymer/clay nanocomposite based on natural silicate montmorilonite (Mt) and a biodegradable polymer polyglycolide (PGA) was prepared and characterized by FT-IR, thermogravimetric analysis, differential thermogravimetric analysis and X-ray diffraction. Then, the resulting matrix was used as a fixation matrix for pyranose oxidase (POx), which was selected as a model enzyme. The bioactive layer was fabricated by immobilization of POx on glassy carbon electrodes by means of PGA-Mt and bovine serum albumin. The POx biosensor revealed a good linear range from 0.01 to 0.5 mM glucose with a LOD of 1.2 μM. After the optimization of the working and preparation conditions, characterization studies were performed for glucose detection. Finally, the PGA-Mt/POx biosensor was confirmed to have detected glucose in beverages without needing any sample pre-treatment.
ISSN: 1144-0546
Appears in Collections:Chemistry / Kimya
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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