Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6325
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dc.contributor.authorAbuzayed, Mazen A.-
dc.contributor.authorGöktay, Mehmet-
dc.contributor.authorAllmer, Jens-
dc.contributor.authorDoğanlar, Sami-
dc.contributor.authorFrary, Anne-
dc.date.accessioned2017-10-10T07:51:59Z-
dc.date.available2017-10-10T07:51:59Z-
dc.date.issued2017-02-
dc.identifier.citationAbuzayed, M. A., Göktay, M., Allmer, J., Doğanlar, S., and Frary, A. (2017). Development of genomic simple sequence repeat markers in faba bean by next-generation sequencing. Plant Molecular Biology Reporter, 35(1), 61-71. doi:10.1007/s11105-016-1003-1en_US
dc.identifier.issn0735-9640-
dc.identifier.issn1556-469X-
dc.identifier.urihttp://doi.org/10.1007/s11105-016-1003-1-
dc.identifier.urihttp://hdl.handle.net/11147/6325-
dc.description.abstractFaba bean (Vicia faba L.) is an important food legume crop with a huge genome. Development of genetic markers for faba bean is important to study diversity and for molecular breeding. In this study, we used Next Generation Sequencing (NGS) technology for the development of genomic simple sequence repeat (SSR) markers. A total of 14,027,500 sequence reads were obtained comprising 4,208 Mb. From these reads, 56,063 contigs were assembled (16,367 Mb) and 2138 SSRs were identified. Mono and dinucleotides were the most abundant, accounting for 57.5 % and 20.9 % of all SSR repeats, respectively. A total of 430 primer pairs were designed from contigs larger than 350 nucleotides and 50 primers pairs were tested for validation of SSR locus amplification. Nearly all (96 %) of the markers were found to produce clear amplicons and to be reproducible. Thirty-nine SSR markers were then applied to 46 faba bean accessions from worldwide origins, resulting in 161 alleles with 87.5 % polymorphism, and an average of 4.1 alleles per marker. Gene diversity (GD) of the markers ranged from 0 to 0.48 with an average of 0.27. Testing of the markers showed that they were useful in determining genetic relationships and population structure in faba bean accessions.en_US
dc.description.sponsorshipRepublic of Turkey's Ministry of Science, Industry and Technology (0424.STZ.2013-2); Scientific and Technological Research Council of Turkey (113E326)en_US
dc.language.isoenen_US
dc.publisherSpringer Verlagen_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/EEEAG/113E326en_US
dc.relation.ispartofPlant Molecular Biology Reporteren_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGenetic diversityen_US
dc.subjectGenomic SSRsen_US
dc.subjectIllumina sequencingen_US
dc.subjectPopulation structureen_US
dc.subjectVicia faba L.en_US
dc.titleDevelopment of genomic simple sequence repeat markers in faba bean by next-generation sequencingen_US
dc.typeArticleen_US
dc.authoridTR107974en_US
dc.authoridTR1977en_US
dc.authoridTR114553en_US
dc.institutionauthorAbuzayed, Mazen A.-
dc.institutionauthorGöktay, Mehmet-
dc.institutionauthorAllmer, Jens-
dc.institutionauthorDoğanlar, Sami-
dc.institutionauthorFrary, Anne-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume35en_US
dc.identifier.issue1en_US
dc.identifier.startpage61en_US
dc.identifier.endpage71en_US
dc.identifier.wosWOS:000394150700005en_US
dc.identifier.scopus2-s2.0-84982290232en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s11105-016-1003-1-
dc.relation.doi10.1007/s11105-016-1003-1en_US
dc.coverage.doi10.1007/s11105-016-1003-1en_US
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.grantfulltextopen-
crisitem.author.dept01. Izmir Institute of Technology-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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