Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6361
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dc.contributor.authorAtik, Ahmet Emin-
dc.contributor.authorGüray, Melda Zeynep-
dc.contributor.authorYalçın, Talat-
dc.date.accessioned2017-10-16T10:52:38Z-
dc.date.available2017-10-16T10:52:38Z-
dc.date.issued2017-03-
dc.identifier.citationAtik, A. E., Güray, M. Z., and Yalçın, T. (2017). Observation of the side chain O-methylation of glutamic acid or aspartic acid containing model peptides by electrospray ionization-mass spectrometry. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1047, 75-83. doi:10.1016/j.jchromb.2016.12.043en_US
dc.identifier.issn1570-0232-
dc.identifier.urihttp://doi.org/10.1016/j.jchromb.2016.12.043-
dc.identifier.urihttp://hdl.handle.net/11147/6361-
dc.description.abstractO-methylation of the side chains of glutamic acid (E) and aspartic acid (D) residues is generally observed modification when an acidified methanol/water (MeOH/dH2O) mixture is used as a solvent system during sample preparation for proteomic research. This chemical modification may result misidentification with endogenous protein methylation; therefore, a special care should be taken during sample handling prior to mass spectrometric analysis. In the current study, we systematically examined the extent of E/D methylation and C-terminus carboxyl group of synthetic model peptides in terms of different incubation temperatures, storage times, and added acid types as well as its percentages. To monitor these effects, C-terminus amidated and free acid forms of synthetic model peptides comprised of E or D residue(s) have been analyzed by electrospray ionization-mass spectrometry (ESI-MS). Additionally, LC–MS/MS experiments were performed to confirm the formation of methylated peptide product. The results showed that the rate of methylation was increased as the temperature increases along with prolong incubation times. Moreover, the extent of methylation was remarkably high when formic acid (FA) used as a protonation agent instead of acetic acid (AA). In addition, it was found that the degree of methylation was significantly decreased by lowering acid percentages in ESI solution. More than one acidic residue containing model peptides have been also used to explore the extent of multiple methylation reaction. Lastly, the ethanol (EtOH) and isopropanol (iPrOH) have been substituted separately with MeOH in sample preparation step to investigate the extent of esterification reaction under the same experimental conditions. However, in the positive perspective of view, this method can be used as a simple, rapid and cheap method for methylation of acidic residues under normal laboratory conditions.en_US
dc.description.sponsorshipThe State Planning Orga-nization (DPT) of Turkey; Izmir Institute of Technology; TUBITAK (113Z172-COST 1306)en_US
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/KBAG/113Z172en_US
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciencesen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAspartic aciden_US
dc.subjectGlutamic aciden_US
dc.subjectO-methylationen_US
dc.subjectElectrospray ionization mass spectrometryen_US
dc.titleObservation of the side chain O-methylation of glutamic acid or aspartic acid containing model peptides by electrospray ionization-mass spectrometryen_US
dc.typeArticleen_US
dc.authoridTR130617en_US
dc.institutionauthorAtik, Ahmet Emin-
dc.institutionauthorGüray, Melda Zeynep-
dc.institutionauthorYalçın, Talat-
dc.departmentİzmir Institute of Technology. Chemistryen_US
dc.identifier.volume1047en_US
dc.identifier.startpage75en_US
dc.identifier.endpage83en_US
dc.identifier.wosWOS:000397686200008en_US
dc.identifier.scopus2-s2.0-85008500705en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.jchromb.2016.12.043-
dc.identifier.pmid28063777en_US
dc.relation.doi10.1016/j.jchromb.2016.12.043en_US
dc.coverage.doi10.1016/j.jchromb.2016.12.043en_US
dc.identifier.wosqualityQ2-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept04.01. Department of Chemistry-
Appears in Collections:Chemistry / Kimya
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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