Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6381
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dc.contributor.authorUz, Metin-
dc.contributor.authorBüyüköz, Melda-
dc.contributor.authorSharma, Anup D.-
dc.contributor.authorSakaguchi, Donald S.-
dc.contributor.authorAlsoy Altınkaya, Sacide-
dc.contributor.authorMallapragada, Surya K.-
dc.date.accessioned2017-10-18T13:36:55Z-
dc.date.available2017-10-18T13:36:55Z-
dc.date.issued2017-04-
dc.identifier.citationUz, M., Büyüköz, M., Sharma, A. D., Sakaguchi, D. S., Alsoy Altınkaya, S., and Mallapragada, S. K. (2017). Gelatin-based 3D conduits for transdifferentiation of mesenchymal stem cells into Schwann cell-like phenotypes. Acta Biomaterialia, 53, 293-306. doi:10.1016/j.actbio.2017.02.018en_US
dc.identifier.issn1742-7061-
dc.identifier.urihttp://doi.org/10.1016/j.actbio.2017.02.018-
dc.identifier.urihttp://hdl.handle.net/11147/6381-
dc.description.abstractIn this study, gelatin-based 3D conduits with three different microstructures (nanofibrous, macroporous and ladder-like) were fabricated for the first time via combined molding and thermally induced phase separation (TIPS) technique for peripheral nerve regeneration. The effects of conduit microstructure and mechanical properties on the transdifferentiation of bone marrow-derived mesenchymal stem cells (MSCs) into Schwann cell (SC) like phenotypes were examined to help facilitate neuroregeneration and understand material-cell interfaces. Results indicated that 3D macroporous and ladder-like structures enhanced MSC attachment, proliferation and spreading, creating interconnected cellular networks with large numbers of viable cells compared to nanofibrous and 2D-tissue culture plate counterparts. 3D-ladder-like conduit structure with complex modulus of ∼0.4 × 106 Pa and pore size of ∼150 μm provided the most favorable microenvironment for MSC transdifferentiation leading to ∼85% immunolabeling of all SC markers. On the other hand, the macroporous conduits with complex modulus of ∼4 × 106 Pa and pore size of ∼100 μm showed slightly lower (∼65% for p75, ∼75% for S100 and ∼85% for S100β markers) immunolabeling. Transdifferentiated MSCs within 3D-ladder-like conduits secreted significant amounts (∼2.5 pg/mL NGF and ∼0.7 pg/mL GDNF per cell) of neurotrophic factors, while MSCs in macroporous conduits released slightly lower (∼1.5 pg/mL NGF and 0.7 pg/mL GDNF per cell) levels. PC12 cells displayed enhanced neurite outgrowth in media conditioned by conduits with transdifferentiated MSCs. Overall, conduits with macroporous and ladder-like 3D structures are promising platforms in transdifferentiation of MSCs for neuroregeneration and should be further tested in vivo. Statement of Significance This manuscript focuses on the effect of microstructure and mechanical properties of gelatin-based 3D conduits on the transdifferentiation of mesenchymal stem cells to Schwann cell-like phenotypes. This work builds on our recently accepted manuscript in Acta Biomaterialia focused on multifunctional 2D films, and focuses on 3D microstructured conduits designed to overcome limitations of current strategies to facilitate peripheral nerve regeneration. The comparison between conduits fabricated with nanofibrous, macroporous and ladder-like microstructures showed that the ladder-like conduits showed the most favorable environment for MSC transdifferentiation to Schwann-cell like phenotypes, as seen by both immunolabeling as well as secretion of neurotrophic factors. This work demonstrates the importance of controlling the 3D microstructure to facilitate tissue engineering strategies involving stem cells that can serve as promising approaches for peripheral nerve regeneration.en_US
dc.description.sponsorshipUS Army Medical Research and Materiel Command (W81XWH-11-1-0700); Stem Cell Biology Fund; Stanley Endowed Chairen_US
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.relation.ispartofActa Biomaterialiaen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject3D gelatin conduitsen_US
dc.subjectNerve regenerationen_US
dc.subjectTransdifferentiationen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectSchwann cellsen_US
dc.titleGelatin-based 3D conduits for transdifferentiation of mesenchymal stem cells into Schwann cell-like phenotypesen_US
dc.typeArticleen_US
dc.authoridTR2091en_US
dc.institutionauthorAlsoy Altınkaya, Sacide-
dc.departmentİzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume53en_US
dc.identifier.startpage293en_US
dc.identifier.endpage306en_US
dc.identifier.wosWOS:000401679100026en_US
dc.identifier.scopus2-s2.0-85013661245en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.actbio.2017.02.018-
dc.identifier.pmid28213098en_US
dc.relation.doi10.1016/j.actbio.2017.02.018en_US
dc.coverage.doi10.1016/j.actbio.2017.02.018en_US
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept03.02. Department of Chemical Engineering-
crisitem.author.dept03.02. Department of Chemical Engineering-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Sürdürülebilir Yeşil Kampüs Koleksiyonu / Sustainable Green Campus Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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