Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6423
Full metadata record
DC FieldValueLanguage
dc.contributor.authorAmuk, Nisa Gül-
dc.contributor.authorKurt, Gökmen-
dc.contributor.authorBaran, Yusuf-
dc.contributor.authorSeyrantepe, Volkan-
dc.contributor.authorKartal Yandım, Melis-
dc.contributor.authorAdan, Aysun-
dc.contributor.authorAkyıldız Demir, Seçil-
dc.contributor.authorKiraz, Yağmur-
dc.contributor.authorSönmez, Mehmet Fatih-
dc.date.accessioned2017-10-30T11:50:41Z
dc.date.available2017-10-30T11:50:41Z
dc.date.issued2017-06
dc.identifier.citationAmuk, N. G., Kurt, G., Baran, Y., Seyrantepe, V., Kartal Yandım, M., Adan, A., Akyıldız Demir, S., Kiraz, Y., and Sönmez, M. F. (2017). Effects of cell-mediated osteoprotegerin gene transfer and mesenchymal stem cell applications on orthodontically induced root resorption of rat teeth. European Journal of Orthodontics, 39(3), 235-242. doi:10.1093/ejo/cjw054en_US
dc.identifier.issn0141-5387
dc.identifier.issn0141-5387-
dc.identifier.urihttp://doi.org/10.1093/ejo/cjw054
dc.identifier.urihttp://hdl.handle.net/11147/6423
dc.description.abstractAim: The aim of this study is to evaluate and compare therapeutic effects of mesenchymal stem cell (MSCs) and osteoprotegerin (OPG) gene transfer applications on inhibition and/or repair of orthodontically induced inflammatory root resorption (OIIRR). Materials and methods: Thirty Wistar rats were divided into four groups as untreated group (negative control), treated with orthodontic appliance group (positive control), MSCs injection group, and OPG transfected MSCs [gene therapy (GT) group]. About 100 g of orthodontic force was applied to upper first molar teeth of rats for 14 days. MSCs and transfected MSC injections were performed at 1st, 6th, and 11th days to the MSC and GT group rats. At the end of experiment, upper first molar teeth were prepared for genetical, scanning electron microscopy (SEM), fluorescent microscopy, and haematoxylin eosin-tartrate resistant acid phosphatase staining histological analyses. Number of total cells, number of osteoclastic cells, number of resorption lacunae, resorption area ratio, SEM resorption ratio, OPG, RANKL, Cox-2 gene expression levels at the periodontal ligament (PDL) were calculated. Paired t-test, Kruskal-Wallis, and chi-square tests were performed. Results: Transferred MSCs showed marked fluorescence in PDL. The results revealed that number of osteoclastic cells, resorption lacunae, resorption area ratio, RANKL, and Cox-2 were reduced after single MSC injections significantly (P < 0.05). GT group showed the lowest number of osteoclastic cells (P < 0.01), number of resorption lacunae, resorption area ratio, and highest OPG expression (P < 0.001). Conclusions: Taken together all these results, MSCs and GT showed marked inhibition and/or repair effects on OIIRR during orthodontic treatment on rats.en_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofEuropean Journal of Orthodonticsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectOsteoprotegerinen_US
dc.subjectGene transferen_US
dc.subjectOrthodontic appliancesen_US
dc.subjectOsteoclastic cellsen_US
dc.subjectTeethen_US
dc.titleEffects of cell-mediated osteoprotegerin gene transfer and mesenchymal stem cell applications on orthodontically induced root resorption of rat teethen_US
dc.typeArticleen_US
dc.authoridTR119193en_US
dc.authoridTR166288en_US
dc.authoridTR144922en_US
dc.institutionauthorBaran, Yusuf-
dc.institutionauthorSeyrantepe, Volkan-
dc.institutionauthorKartal Yandım, Melis-
dc.institutionauthorAdan, Aysun-
dc.institutionauthorAkyıldız Demir, Seçil-
dc.institutionauthorKiraz, Yağmur-
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume39en_US
dc.identifier.issue3en_US
dc.identifier.startpage235en_US
dc.identifier.endpage242en_US
dc.identifier.wosWOS:000407225000002
dc.identifier.scopusSCOPUS:2-s2.0-85027562306
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1093/ejo/cjw054-
dc.relation.doi10.1093/ejo/cjw054en_US
dc.coverage.doi10.1093/ejo/cjw054en_US
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextWith Fulltext-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
crisitem.author.deptDepartment of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Files in This Item:
File Description SizeFormat 
6423.pdfMakale1.3 MBAdobe PDFThumbnail
View/Open
Show simple item record

CORE Recommender

SCOPUSTM   
Citations

8
checked on Dec 4, 2021

WEB OF SCIENCETM
Citations

7
checked on Dec 4, 2021

Page view(s)

32
checked on Dec 6, 2021

Download(s)

32
checked on Dec 6, 2021

Google ScholarTM

Check

Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.