Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6504
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dc.contributor.authorFrary, Anne-
dc.contributor.authorVan Eck, Joyce-
dc.date.accessioned2017-11-27T10:45:34Z
dc.date.available2017-11-27T10:45:34Z
dc.date.issued2005
dc.identifier.citationFrary, A., and Van Eck, J. (2005). Organogenesis from transformed tomato explants. Methods in Molecular Biology, 286, 141-150.en_US
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/11147/6504
dc.description.abstractTomato was one of the first crops for which a genetic transformation system was reported involving regeneration by organogenesis from Agrobacterium-transformed explants. Since the initial reports, various factors have been studied that affect the efficiency of tomato transformation and the technique has been useful for the isolation and identification of many genes involved in plant disease resistance, morphology and development. In this method, cotyledon explants from in vitro-grown seedlings are precultured overnight on a tobacco suspension feeder layer. The explants are then inoculated with Agrobacterium and returned to the feeder layer for a 2-d period of cocultivation. After cocultivation, the explants are transferred to an MS-based selective regeneration medium containing zeatin. Regenerated shoots are then rooted on a separate selective medium. This protocol has been used with several tomato cultivars and routinely yields transformation efficiencies of 10-15%.en_US
dc.language.isoenen_US
dc.publisherHumana Pressen_US
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTomatoen_US
dc.subjectGenetic transformationen_US
dc.subjectLycopersicon esculentumen_US
dc.subjectTransgenic planten_US
dc.subjectOrganogenesisen_US
dc.titleOrganogenesis from transformed tomato explantsen_US
dc.typeArticleen_US
dc.authoridTR114553en_US
dc.institutionauthorFrary, Anne-
dc.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume286en_US
dc.identifier.startpage141en_US
dc.identifier.endpage150en_US
dc.identifier.scopus2-s2.0-16544378940en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.pmid15310918en_US
dc.identifier.scopusqualityQ3-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
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