Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/6718
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dc.contributor.authorÇalışkan, Ceyda-
dc.contributor.authorPehlivan, Melek-
dc.contributor.authorYüce, Zeynep-
dc.contributor.authorSercan, Ogün-
dc.date.accessioned2018-01-22T08:35:42Z
dc.date.available2018-01-22T08:35:42Z
dc.date.issued2017-10
dc.identifier.citationÇalışkan, C., Pehlivan, M., Yüce, Z., and Sercan, O. (2017). Dishevelled proteins and CYLD reciprocally regulate each other in CML cell lines. Molecular Biology Reports, 44(5), 391-397. doi:10.1007/s11033-017-4122-3en_US
dc.identifier.issn0301-4851
dc.identifier.issn0301-4851-
dc.identifier.issn1573-4978-
dc.identifier.urihttp://doi.org/10.1007/s11033-017-4122-3
dc.identifier.urihttp://hdl.handle.net/11147/6718
dc.description.abstractDishevelled (Dvl) proteins are activated by Wnt pathway stimulation and have crucial roles in the regulation of β-catenin destruction complex. CYLD is a tumor suppressor and a deubiquitination enzyme. CYLD negatively regulates the Wnt/β-catenin signaling pathway by deubiquitinating Dvl proteins. Loss of function and mutations of CYLD were linked to different types of solid tumors. Loss of function in CYLD is associated with Dvl hyper ubiquitination, resulting in the transmission of Wnt signaling to downstream effectors. β-catenin upregulation is observed during disease progression in chronic myeloid leukemia (CML). Deregulated Dvl signaling may be a reason for β-catenin activation in CML; and CYLD may contribute to Dvl deregulation. First, we evaluated mRNA expression in three CML cell lines and mRNA expression of the CYLD gene was found to be present in all (K562, MEG01, KU812). Unlike solid tumors sequencing revealed no mutations in the coding sequences of the CYLD gene. DVL genes were silenced by using a pool of siRNA oligonucleotides and gene expression differences in CYLD was determined by RT-PCR and western blot. CYLD protein expression decreased after Dvl silencing. An opposite approach of overexpressing Dvl proteins resulted in upregulated CYLD expression. While previous reports have described CYLD as a regulator of DVL proteins; our data suggests the presence of a more complicated reciprocal regulatory mechanism in CML cell lines.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK 114Z225)en_US
dc.language.isoenen_US
dc.publisherSpringer Verlagen_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChronic myeloid leukemiaen_US
dc.subjectDishevelleden_US
dc.subjectWnt signalingen_US
dc.subjectGene silencingen_US
dc.titleDishevelled proteins and CYLD reciprocally regulate each other in CML cell linesen_US
dc.typeArticleen_US
dc.institutionauthorÇalışkan, Ceyda-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume44en_US
dc.identifier.issue5en_US
dc.identifier.startpage391en_US
dc.identifier.endpage397en_US
dc.identifier.wosWOS:000412913200002en_US
dc.identifier.scopus2-s2.0-85028324890en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s11033-017-4122-3-
dc.identifier.pmid28840581en_US
dc.relation.doi10.1007/s11033-017-4122-3en_US
dc.coverage.doi10.1007/s11033-017-4122-3en_US
dc.identifier.wosqualityQ3-
dc.identifier.scopusqualityQ2-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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