Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/7072
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dc.contributor.authorErdoğan, İpek-
dc.contributor.authorCoşacak, Mehmet İlyas-
dc.contributor.authorNalbant, Ayten-
dc.contributor.authorAkgül, Bünyamin-
dc.date.accessioned2019-01-02T07:02:45Z-
dc.date.available2019-01-02T07:02:45Z-
dc.date.issued2018-
dc.identifier.citationErdoğan, İ., CoşacaK, M. İ., Nalbant, A., and Akgül, B. (2018). Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. Turkish Journal of Biology, 42(2), 113-122. doi:10.3906/biy-1710-62en_US
dc.identifier.issn1300-0152-
dc.identifier.issn1303-6092-
dc.identifier.urihttp://doi.org/10.3906/biy-1710-62-
dc.identifier.urihttp://hdl.handle.net/11147/7072-
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/349094-
dc.description.abstractMicroRNAs (miRNAs) are small noncoding RNAs of about 19-25 nt that regulate gene expression posttranscriptionally under various cellular conditions, including apoptosis. The miRNAs involved in modulation of apoptotic events in T cells are partially known. However, heterogeneity associated with cell lines makes it difficult to interpret gene expression signatures, especially in cancer-related cell lines. Treatment of the Jurkat T-cell leukemia cell line with the universal apoptotic drug, camptothecin, resulted in identification of two Jurkat subpopulations: one that is sensitive to camptothecin and another that is rather intrinsically resistant. We sorted apoptotic Jurkat cells from nonapoptotic ones prior to profiling miRNAs through deep sequencing. Our data showed that a total of 184 miRNAs were dysregulated. Interestingly, the apoptotic and nonapoptotic subpopulations exhibited distinct miRNA expression profiles. In particular, 6 miRNAs were inversely expressed in these two subpopulations. The pyrosequencing results were validated by real-time qPCR. Altogether, these results suggest that miRNAs modulate apoptotic events in T cells and that cellular heterogeneity requires careful interpretation of miRNA expression profiles obtained from drug-treated cell lines.en_US
dc.description.sponsorshipİYTE BİYOMER and TUBITAK (grant 107T475)en_US
dc.language.isoenen_US
dc.publisherTUBITAKen_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TBAG/107T475en_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosisen_US
dc.subjectDeep sequencingen_US
dc.subjectJurkaten_US
dc.subjectMicroRNAsen_US
dc.titleDeep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosisen_US
dc.typeArticleen_US
dc.authorid0000-0003-0266-8306en_US
dc.authorid0000-0001-9877-9689en_US
dc.institutionauthorErdoğan, İpek-
dc.institutionauthorCoşacak, Mehmet İlyas-
dc.institutionauthorNalbant, Ayten-
dc.institutionauthorAkgül, Bünyamin-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume42en_US
dc.identifier.issue2en_US
dc.identifier.startpage113en_US
dc.identifier.endpage122en_US
dc.identifier.wosWOS:000433481900002en_US
dc.identifier.scopus2-s2.0-85046037158en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.3906/biy-1710-62-
dc.identifier.pmid30814873en_US
dc.relation.doi10.3906/biy-1710-62en_US
dc.coverage.doi10.3906/biy-1710-62en_US
dc.identifier.trdizinid349094en_US
dc.identifier.wosqualityQ4-
dc.identifier.scopusqualityQ3-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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