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Title: Transcriptomic analysis of boron hyperaccumulation mechanisms in Puccinellia distans
Authors: Öztürk, Saniye Elvan
Göktay, Mehmet
Has, Canan
Babaoğlu, Mehmet
Allmer, Jens
Doğanlar, Sami
Frary, Anne
Keywords: Bioremediation
Puccinellia distans
RNA sequencing
Issue Date: May-2018
Publisher: Elsevier Ltd.
Source: Öztürk, S. E., Göktay, M., Has, C., Babaoğlu, M., Allmer, J., Doğanlar, S., and Frary, A. (2018). Transcriptomic analysis of boron hyperaccumulation mechanisms in Puccinellia distans. Chemosphere, 199, 390-401. doi:10.1016/j.chemosphere.2018.02.070
Abstract: Puccinellia distans, common alkali grass, is found throughout the world and can survive in soils with boron concentrations that are lethal for other plant species. Indeed, P. distans accumulates very high levels of this element. Despite these interesting features, very little research has been performed to elucidate the boron tolerance mechanism in this species. In this study, P. distans samples were treated for three weeks with normal (0.5 mg L−1) and elevated (500 mg L−1) boron levels in hydroponic solution. Expressed sequence tags (ESTs) derived from shoot tissue were analyzed by RNA sequencing to identify genes up and down-regulated under boron stress. In this way, 3312 differentially expressed transcripts were detected, 67.7% of which were up-regulated and 32.3% of which were down-regulated in boron-treated plants. To partially confirm the RNA sequencing results, 32 randomly selected transcripts were analyzed for their expression levels in boron-treated plants. The results agreed with the expected direction of change (up or down-regulation). A total of 1652 transcripts had homologs in A. thaliana and/or O. sativa and mapped to 1107 different proteins. Functional annotation of these proteins indicated that the boron tolerance and hyperaccumulation mechanisms of P. distans involve many transcriptomic changes including: alterations in the malate pathway, changes in cell wall components that may allow sequestration of excess boron without toxic effects, and increased expression of at least one putative boron transporter and two putative aquaporins. Elucidation of the boron accumulation mechanism is important in developing approaches for bioremediation of boron contaminated soils.
ISSN: 0045-6535
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Sürdürülebilir Yeşil Kampüs Koleksiyonu / Sustainable Green Campus Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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