Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/7760
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dc.contributor.authorPolatoğlu, İlker-
dc.contributor.authorÇakıcıoğlu Özkan, Fehime-
dc.date.accessioned2020-05-07T10:34:49Z
dc.date.available2020-05-07T10:34:49Z
dc.date.issued2019-07en_US
dc.identifier.citationPolatoğlu, İ., and Çakıcıoğlu Özkan, F. (2019). Development of an optical tyrosinase biosensor (TCA) for detection of “Parathion-Methyl”. Sensor Review, 39(4), 604-611. doi:10.1108/SR-10-2018-0242en_US
dc.identifier.issn0260-2288
dc.identifier.issn0260-2288-
dc.identifier.urihttps://doi.org/10.1108/SR-10-2018-0242
dc.identifier.urihttps://hdl.handle.net/11147/7760
dc.description.abstractPurpose: This paper aims to present a novel and cost-effective optical biosensor design by simple preparation method for detection of “parathion-methyl,” which is a model pesticide pose to public health and the environment. Design/methodology/approach: The optical enzyme biosensor (TCA) for detection of pesticide “parathion-methyl” was developed on the basis of immobilization of tyrosinase enzyme on chitosan film by adsorption technique. The analytic performance of TCA was investigated by measuring its activity with Ultraviolet (UV) visible spectrophotometer. Findings: Uniform porous network structure and protonated groups of chitosan film provided a microenvironment for tyrosinase immobilization evident from Fourier transform infrared (FTIR) spectroscopy and Atomic Force Microscopy analysis. TCA has a wide linear detection range (0-1.03 µM) with high correlation coefficient and it can detect the parathion-methyl concentration as low as 159 nM by noncompetitive inhibition kinetics. Using the TCA sensor both for ten times and at least 45 days without a significant loss in its activity are the indicators of its good operational and storage stability. Moreover, TCA can be applicable to tap water, providing a promising tool for pesticides detection. Originality/value: This is the first time to use the in situ analytical technique that can improve the performance of optical enzyme sensor provided to control the pesticide residue better with respect to traditional techniques. The effect of organic solvents on the performance of optical enzyme biosensor was investigated. Inhibition kinetic of the solvents rarely encountered in literature was also studied besides the pH and temperature tolerance of the optical biosensor.en_US
dc.language.isoenen_US
dc.publisherEmerald Group Publishing Ltd.en_US
dc.relation.ispartofSensor Reviewen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAdsorptionen_US
dc.subjectOrganophosphate pesticideen_US
dc.subjectParathion-methylen_US
dc.subjectTyrosinase biosensoren_US
dc.subjectBiosensorsen_US
dc.titleDevelopment of an optical tyrosinase biosensor (TCA) for detection of “Parathion-Methyl”en_US
dc.typeArticleen_US
dc.institutionauthorÇakıcıoğlu Özkan, Fehime-
dc.departmentİzmir Institute of Technology. Chemical Engineeringen_US
dc.identifier.volume39en_US
dc.identifier.issue4en_US
dc.identifier.startpage604en_US
dc.identifier.endpage611en_US
dc.identifier.wosWOS:000486000400018en_US
dc.identifier.scopus2-s2.0-85067887451en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1108/SR-10-2018-0242-
dc.relation.doi10.1108/SR-10-2018-0242en_US
dc.coverage.doi10.1108/SR-10-2018-0242en_US
dc.identifier.wosqualityQ4-
dc.identifier.scopusqualityQ2-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Sürdürülebilir Yeşil Kampüs Koleksiyonu / Sustainable Green Campus Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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