Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/8840
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dc.contributor.authorKaci, Fatma Necmiye-
dc.contributor.authorKiraz, Yağmur-
dc.contributor.authorÇekdemir, Demet-
dc.contributor.authorBaran, Yusuf-
dc.date.accessioned2020-07-18T08:34:02Z-
dc.date.available2020-07-18T08:34:02Z-
dc.date.issued2020-
dc.identifier.issn0188-4409-
dc.identifier.issn1873-5487-
dc.identifier.urihttps://doi.org/10.1016/j.arcmed.2020.01.012-
dc.identifier.urihttps://hdl.handle.net/11147/8840-
dc.description.abstractBackground. In this study, we aimed to determine synergistic apoptotic and cytotoxic effects of methylstat and bortezomib on U266 and ARH77 multiple myeloma (MM) cells. Methods. Cytotoxic effects of the drugs were demonstrated by MTT cell proliferation assay while apoptotic effects were examined by loss of mitochondrial membrane potential (MMP) by JC-1 MMP detection kit, changes in caspase-3 enzyme activity and Annexin-V apoptosis assay by flow cytometry. Expression levels of apoptotic and antiapoptotic genes were examined by qRT-PCR. Results. Our results showed that combination of methylstat and bortezomib have synergistic antiproliferative effect on MM cells as compared to either agent alone. These results were also confirmed by showing synergistic apoptotic effects determined by increased loss of mitochondrial membrane potential and increased caspase-3 enzyme activity and relocation of phosphotidyleserine on the cell membrane by Annexin-V/PI double staining. Combination of bortezomib with methylstat arrested cells at the S phase of the cell cycle. Methylstat treatment caused upregulation of FASLG, NGFR, TNF, TNI-RS10B and TNFRS1B apoptotic genes and downregulation of AKT1, AVEN, BAG1 BCL2L2 and RELA antiapoptotic genes in a dose and time dependent manner. Conclusion. In conclusion, our data suggested that bortezomib in combination with methylstat decreased cell proliferation and induced apoptosis significantly in U266 and ARH77 cells. When supported with in vivo analyses, methylstat might be considered as a potential new agent for the treatment of MM. (C) 2020 IMSS. Published by Elsevier Inc.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofArchives of Medical Researchen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMultiple myelomaen_US
dc.subjectBortezomiben_US
dc.subjectMethylstaten_US
dc.subjectApoptosisen_US
dc.titleSynergistic apoptotic effects of bortezomib and methylstat on multiple myeloma cellsen_US
dc.typeArticleen_US
dc.institutionauthorKaci, Fatma Necmiye-
dc.institutionauthorKiraz, Yağmur-
dc.institutionauthorBaran, Yusuf-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume51en_US
dc.identifier.issue3en_US
dc.identifier.startpage187en_US
dc.identifier.endpage193en_US
dc.identifier.wosWOS:000536139200001en_US
dc.identifier.scopus2-s2.0-85080036933en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.arcmed.2020.01.012-
dc.identifier.pmid32111493en_US
dc.relation.doi10.1016/j.arcmed.2020.01.012en_US
dc.coverage.doi10.1016/j.arcmed.2020.01.012en_US
dc.identifier.wosqualityQ4-
dc.identifier.scopusqualityQ2-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.openairetypeArticle-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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