Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/9523
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dc.contributor.authorAnıl İnevi, Müge-
dc.contributor.authorSağlam Metiner, Pelin-
dc.contributor.authorKabak, Evrim Ceren-
dc.contributor.authorGülce İz, Sultan-
dc.date.accessioned2020-07-25T22:16:52Z-
dc.date.available2020-07-25T22:16:52Z-
dc.date.issued2020-
dc.identifier.issn0301-4851-
dc.identifier.issn1573-4978-
dc.identifier.urihttps://doi.org/10.1007/s11033-019-05111-z-
dc.identifier.urihttps://hdl.handle.net/11147/9523-
dc.description.abstractBreast cancer is one of the most common cancer types among women in which early tumor invasion leads to metastases and death. EpCAM (epithelial cellular adhesion molecule) and HER2 (human epidermal growth factor receptor 2) are two main circulating tumor cell (CTC) subsets in HER2+ breast cancer patients. In this regard, the main aim of this study is to develop and characterize a three-dimensional (3D) breast cancer tumor model composed of CTC subsets to evaluate new therapeutic strategies and drugs. For this reason, EpCAM(+) and HER2(+) sub-populations were isolated from different cell lines to establish 3D tumor model that mimics in situ (in vivo) more closely than two-dimensional (2D) models. EpCAM(+)/HER2(+) cells had a high proliferation rate and low tendency to attach to the surface in comparison with parental MDA-MB-453 cells as CTC subsets. Aggressive breast cancer subpopulations cultured in 3D porous chitosan scaffold had enhanced cell-cell and cell-matrix interactions compared to 2D cultured cells and these 3D models showed more aggressive morphology and behavior, expressed higher levels of pluripotency marker genes, Nanog, Sox2 and Oct4. For the verification of the 3D model, the effects of doxorubicin which is a chemotherapeutic agent used in breast cancer treatment were examined and increased drug resistance was determined in 3D cultures. The 3D tumor model comprising EpCAM(+)/HER2(+) CTC subsets developed in this study has a promising potential to be used for investigation of an aggressive CTC microenvironment in vitro that mimics in vivo characteristics to test new drug candidates against CTCs.en_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHER2en_US
dc.subjectEpCAMen_US
dc.subjectBreast canceren_US
dc.subjectCTCsen_US
dc.subjectCirculating tumor cellsen_US
dc.subject3D tumor modelen_US
dc.titleDevelopment and verification of a three-dimensional (3D) breast cancer tumor model composed of circulating tumor cell (CTC) subsetsen_US
dc.typeArticleen_US
dc.institutionauthorAnıl-İnevi, Müge-
dc.departmentİzmir Institute of Technology. Bioengineeringen_US
dc.identifier.volume47en_US
dc.identifier.issue1en_US
dc.identifier.startpage97en_US
dc.identifier.endpage109en_US
dc.identifier.wosWOS:000489549500001en_US
dc.identifier.scopus2-s2.0-85073939108en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s11033-019-05111-z-
dc.identifier.pmid31583566en_US
dc.relation.doi10.1007/s11033-019-05111-zen_US
dc.coverage.doi10.1007/s11033-019-05111-zen_US
dc.identifier.wosqualityQ4-
dc.identifier.scopusqualityQ3-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
crisitem.author.dept03.01. Department of Bioengineering-
Appears in Collections:Bioengineering / Biyomühendislik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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