Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/9544
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dc.contributor.authorBalkan, Çiğdem-
dc.contributor.authorErcan, İlkcan-
dc.contributor.authorIşık, Esin-
dc.contributor.authorAkdeniz, Esra Şahin-
dc.contributor.authorBalcıoğlu, Orhan-
dc.contributor.authorKodedova, Marie-
dc.contributor.authorKoç, Ahmet-
dc.date.accessioned2020-07-25T22:16:54Z-
dc.date.available2020-07-25T22:16:54Z-
dc.date.issued2019-
dc.identifier.issn0066-4804-
dc.identifier.issn1098-6596-
dc.identifier.urihttps://doi.org/10.1128/AAC.02268-18-
dc.identifier.urihttps://hdl.handle.net/11147/9544-
dc.description.abstractThere are only a few antifungal drugs used systemically in treatment, and invasive fungal infections that are resistant to these drugs are an emerging problem in health care. In this study, we performed a high-copy-number genomic DNA (gDNA) library screening to find and characterize genes that reduce susceptibility to amphotericin B, caspofungin, and voriconazole in Saccharomyces cerevisiae. We identified the PDR16 and PMP3 genes for amphotericin B, the RMD9 and SWH1 genes for caspofungin, and the MRS3 and TRI1 genes for voriconazole. The deletion mutants for PDR16 and PMP3 were drug susceptible, but the other mutants had no apparent susceptibility. Quantitative-PCR analyses suggested that the corresponding drugs upregulated expression of the PDR16, PMP3, SWH1, and MRS3 genes. To further characterize these genes, we also profiled the global expression patterns of the cells after treatment with the antifungals and determined the genes and paths that were up-or downregulated. We also cloned Candida albicans homologs of the PDR16, PMP3, MRS3, and TRI1 genes and expressed them in S. cerevisiae. Heterologous expression of Candida homologs also provided reduced drug susceptibility to the budding yeast cells. Our analyses suggest the involvement of new genes in antifungal drug resistance.en_US
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofAntimicrobial Agents and Chemotherapyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAmphotericin Ben_US
dc.subjectAntifungal agentsen_US
dc.subjectCaspofunginen_US
dc.subjectDrug resistanceen_US
dc.subjectGenomicsen_US
dc.subjectMultidrug resistanceen_US
dc.titleGenomewide elucidation of drug resistance mechanisms for systemically used antifungal drugs amphotericin B, caspofungin, and voriconazole in the budding yeasten_US
dc.typeArticleen_US
dc.institutionauthorBalkan, Çiğdem-
dc.institutionauthorErcan, İlkcan-
dc.institutionauthorIşık, Esin-
dc.institutionauthorAkdeniz, Esra Şahin-
dc.institutionauthorBalcıoğlu, Orhan-
dc.institutionauthorKoç, Ahmet-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume63en_US
dc.identifier.issue9en_US
dc.identifier.wosWOS:000483118000004en_US
dc.identifier.scopus2-s2.0-85071238873en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1128/AAC.02268-18-
dc.identifier.pmid31209012en_US
dc.relation.doi10.1128/AAC.02268-18en_US
dc.coverage.doi10.1128/AAC.02268-18en_US
local.message.claim2022-06-06T11:59:56.890+0300|||rp00417|||submit_approve|||dc_contributor_author|||None*
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.openairetypeArticle-
item.cerifentitytypePublications-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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