Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/9567
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dc.contributor.authorAkyıldız Demir, Seçil-
dc.contributor.authorSeyrantepe, Volkan-
dc.date.accessioned2020-07-25T22:16:56Z-
dc.date.available2020-07-25T22:16:56Z-
dc.date.issued2019-
dc.identifier.issn0250-4685-
dc.identifier.issn1303-829X-
dc.identifier.urihttps://doi.org/10.1515/tjb-2018-0089-
dc.identifier.urihttps://hdl.handle.net/11147/9567-
dc.description.abstractBackground: Cytoplasmic sialidase (NEU2) plays an active role in removing sialic acids from oligosaccharides, gly-copeptides, and gangliosides in mammalian cells. NEU2 is involved in various cellular events, including cancer metabolism, neuronal and myoblast differentiation, proliferation, and hypertrophy. However, NEU2-interacting protein(s) within the cell have not been identified yet. Objective: The aim of this study is to investigate NEU2 interacting proteins using two-step affinity purification (TAP) strategy combined with mass spectrometry analysis. Methods: In this study, NEU2 gene was cloned into the pCTAP expression vector and transiently transfected to COS-7 cells by using PEI. The most efficient expression time of NEU2- tag protein was determined by real-time PCR and Western blot analysis. NEU2-interacting protein(s) were investigated by using TAP strategy combined with two different mass spectrometry experiment; LC-MS/MS and MALDI TOF/TOF. Results: Here, mass spectrometry analysis showed four proteins; a-actin, beta-actin, calmodulin and histone H1.2 proteins are associated with NEU2. The interactions between NEU2 and actin filaments were verified by Western blot analysis and immunofluorescence analysis. Conclusions: Our study suggests that association of NEU2 with actin filaments and other protein(s) could be important for understanding the biological role of NEU2 in mammalian cells.en_US
dc.language.isoenen_US
dc.publisherTürk Biyokimya Derneğien_US
dc.relation.ispartofTurkish Journal of Biochemistryen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSialidaseen_US
dc.subjectNEU2en_US
dc.subjectActinen_US
dc.subjectCalmodulinen_US
dc.subjectStreptavidinen_US
dc.titleIdentification of cytoplasmic sialidase NEU2-associated proteins by LC-MS/MSen_US
dc.title.alternativeSitoplazmik sialidaz NEU2 ile ilişkili proteinlerin LC-MS/MS ile tanımlanması-
dc.typeArticleen_US
dc.institutionauthorDemir, Seçil Akyıldız-
dc.institutionauthorSeyrantepe, Volkan-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume44en_US
dc.identifier.issue4en_US
dc.identifier.startpage462en_US
dc.identifier.endpage472en_US
dc.identifier.wosWOS:000489303900006en_US
dc.identifier.scopus2-s2.0-85073574385en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1515/tjb-2018-0089-
dc.relation.doi10.1515/tjb-2018-0089en_US
dc.coverage.doi10.1515/tjb-2018-0089en_US
dc.identifier.trdizinid340492en_US
dc.identifier.wosqualityQ4-
dc.identifier.scopusqualityQ4-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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