Yıldız, Ahu ArslanArslan Yıldız, AhuOnbaş, Rabia03.01. Department of Bioengineering03. Faculty of Engineering01. Izmir Institute of Technology2023-10-032023-10-0320231937-33411937-335Xhttps://hdl.handle.net/11147/13790Tissue Engineering and Regenerative Medicine International Society (TERMIS) -- JUN 28-JUL 01, 2022 -- Krakow, POLANDSpheroids are one of the well-characterized 3D cell culture approaches for drug screening and therapeutic studies. Magnetic levitation (MagLev) is a newly developing approach to form 3D cellular structures and spheroids [1,2,3]. Magnetic levitational assembly of cells provides rapid, simple, cost-effective 3D cell culture formation while ensuring scaffold-free microenvironment. Here, our efforts are summarized in designing new magnetic levitation platform and biofabrication of 3D cellular entities via magnetic levitation for tissue engineering. Magnetic levitation and guidance of cells were provided by using a paramagnetic agent to fabricate scaffold-free 3D cellular structures. The parameters of cell density, paramagnetic agent concentration, and culturing time were optimized to obtain 3D cardiac cellular structures with tunable size, circularity, and high cell viability. Cellular and extracellular components of the 3D cellular structures were demonstrated via immunofluorescent staining. Also, 3D cardiac cellular structures showed more resistance to drug exposure compared to 2D control. In conclusion, MagLev methodology offers an easy and efficient way to fabricate 3D cellular structures for drug screening studies.eninfo:eu-repo/semantics/closedAccessMagnetic levitationScaffold-free tissue engineering3D cell cultureDrug screening assaysConference Object