Doğdu Okçu, GamzeÖkten, Hatice EserYalçuk, Arda03.07. Department of Environmental Engineering03. Faculty of Engineering01. Izmir Institute of Technology2020-06-292020-06-292020-03Doğdu Okçu, G., Ökten, H. E., and Yalçuk, A. (2020). A hybrid process for 2,4-dichlorophenoxy acetic acid herbicidal treatment and its microbial identification by MALDI-TOF mass spectrometry. Environmental Technology, 41(6), 764-776. doi:10.1080/09593330.2018.15104330959-3330https://doi.org/10.1080/09593330.2018.1510433https://hdl.handle.net/11147/7775The feasibility of coupling photocatalysis and a biological treatment to remove a herbicide–2,4-dichlorophenoxy acetic acid (2,4-D)–from pure water was examined using batch experiments following three protocols: aerated (A-BR) and non-aerated biodegradation (NA-BR) alone, and intimately combined photodegradation and biodegradation (P-B). In view of a subsequent biological treatment, 15 and 180 min irradiation times were chosen in accordance with spectrophotometric and LC-MS/MS results that indicated the decrease in the COD/TOC ratio during photocatalysis. Pre-treatment led to a quick decrease in concentration of 2,4-D and COD during the biological process: a 78.79 ± 0.30% COD removal and 38.23 ± 3.12% 2,4-D elimination was measured after 5760 min in A-BR, and 80.89 ± 0.81% COD and 81.36 ± 1.37% 2,4-D removal was achieved after 2880 min in P-B. For species identification using matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF)-TOF/MS equipment, Aeromonas eucrenophila, Stenotrophomonas acidaminiphila, Ralstonia pickettii, Sphingobacterium multivorum and Acinetobacter towneri were identified with high accuracy, and they play important roles in the degradation of 2,4-D.eninfo:eu-repo/semantics/openAccessAcetic acidBiodegradabilityBiological treatmentMALDI-TOF-MSPhotocatalysisArticle2-s2.0-8505295040110.1080/09593330.2018.151043310.1080/09593330.2018.1510433