Svetlakova, AnnaCerna, HanaNovak, JanŞelale, Hatice04.03. Department of Molecular Biology and Genetics04. Faculty of Science01. Izmir Institute of Technology2020-07-252020-07-252016978-80-7509-443-8https://hdl.handle.net/11147/946723rd International PhD Students Conference (MendelNet) -- NOV 09-10, 2016 -- Mendel Univ Brno, Fac AgriSciences, Brno, CZECH REPUBLICWOS: 000392968500183Nuclear proteome contains important regulatory proteins. To improve the detection of these proteins, Percoll gradient-based fractionation techniques have been developed and optimized. However, owing to the ever increasing sensitivity of identification methods based on liquid chromatography and mass spectrometry, the time and material consuming fractionation methods may no longer be necessary. Here, we show that a Percoll-based nuclear protein fractionation of tomato leaf proteome increased the number of detected proteins, but at least some nuclear proteins were lost or depleted in the process.eninfo:eu-repo/semantics/openAccessNuclear proteomeLC-MSProteome fractionationConference Object