Bor, GizemÜçüncü, MuhammedEmrullahoğlu, MustafaTomak, AyselŞanlı Mohamed, Gülşah2017-07-242017-07-242017Bor, G., Üçüncü, M., Emrullahoǧlu, M., Tomak, A., and Şanlı Mohamed, G. (2016). BODIPY-conjugated chitosan nanoparticles as a fluorescent probe. Drug and Chemical Toxicology. doi:10.1080/01480545.2016.12384810148-05450148-05451525-6014http://doi.org/10.1080/01480545.2016.1238481https://hdl.handle.net/11147/5998Recently, development of fluorescent nanoparticle-based probes for various bioimaging applications has attracted great attention. This work aims to develop a new type fluorescent nanoparticle conjugate and evaluate its cytotoxic effects on A549 and BEAS 2B cell lines. Throughout the study, ionically crosslinked chitosan nanoparticles (CNs) were conjugated with carboxylated 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY-COOH). The results of conjugates (BODIPY-CNs) were investigated with regard to their physic-chemical, optical, cytotoxic properties and cellular internalization. The morphology of BODIPY-CNs was found to be spherical in shape and quite uniform having average diameter of 70.25 ± 11.99 nm. Cytotoxicty studies indicated that although BODIPY-COOH itself was quite toxic on both A549- and BEAS 2B-treated cells, CNs increased the cell viability of both cell lines via conjugation to BODIPY-COOH fluorescent molecule up to 67% for A549 and 74% for BEAS 2B cells. These results may suggest a possible utilization of the new fluorescent nanoparticle-based probe for bioimaging in biology and medicine.eninfo:eu-repo/semantics/openAccessA549 cellsBEAS 2B cellsBODIPYChitosanCytotoxicityArticle2-s2.0-8499649041110.1080/01480545.2016.1238481