Molva, MuratKılıç, SevgiÖzdemir, Ekrem2017-06-152017-06-152016Molva, M., Kılıç, S., and Özdemir, E. (2016). Effect of carbonic anhydrase on CaCO3 crystallization in alkaline solution. Energy and Fuels, 30(12), 10686-10695. doi:10.1021/acs.energyfuels.6b024750887-06241520-5029http://doi.org/10.1021/acs.energyfuels.6b02475https://hdl.handle.net/11147/5772The effect of bovine carbonic anhydrase (CA) on calcium carbonate (CaCO3) crystallization was investigated. A new method was developed to estimate the biocatalytic activity of CA in alkaline solution. The CA was immobilized within polyurethane (PU) foam, and compared its biocatalytic activity with the free-CA and bare-PU foam. A minireactor was created in a calcium hydroxide (Ca(OH)2) solution in order to control the CO2 transfer rate, and reproducible results were obtained. It was found that the free-CA lost its activity in less than 6 min at pH 12.5 in alkaline Ca(OH)2 solution. The CaCO3 crystallization rates for the immobilized-CA were found to be a U-shape, relatively lower at lower immobilized-CA amounts compared to those for the free-CA and bare-PU foam and higher when the amount of immobilized-CA increased. It was concluded that a higher immobilized-CA amount is required to accelerate the CaCO3 crystallization rates in Ca(OH)2 solution.eninfo:eu-repo/semantics/openAccessCarbonic anhydraseAlkalinityCalcium carbonateCrystallization kineticsPolyurethane foamsArticle2-s2.0-8500650859210.1021/acs.energyfuels.6b0247510.1021/acs.energyfuels.6b02475