Bozdağ, Gönensin OzanKaya, AlaattinKoç, AhmetNoll, Gundula A.Prüfer, DirkKarakaya, Hüseyin Çağlar2017-05-172017-05-172014Bozdağ, G.O., Kaya, A., Koç, A., Noll, G.A., Prüfer, D., and Karakaya, H.Ç. (2014). Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast. Gene, 538(2), 251-257. doi:10.1016/j.gene.2014.01.0520378-1119https://doi.org/10.1016/j.gene.2014.01.052https://hdl.handle.net/11147/5530Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.eninfo:eu-repo/semantics/openAccessBeta maritimaHeavy metal toxicityIon homeostasisNickelTransporter proteinYeastArticle2-s2.0-8489390212910.1016/j.gene.2014.01.05210.1016/j.gene.2014.01.052