Evaluation of Agro-Industrial Wastes, Their State, and Mixing Ratio for Maximum Polygalacturonase and Biomass Production in Submerged Fermentation

Göğüş, NihanEvcan, EzgiEvcan, EzgiTarı, CananTarı, CananCavalitto, Sebastian F.03.08. Department of Food Engineering03. Faculty of Engineering01. Izmir Institute of Technology2017-06-012017-06-012015-10Göğüş, N., Evcan, E., Tarı, C., and Cavalitto, S. F. (2015). Evaluation of agro-industrial wastes, their state, and mixing ratio for maximum polygalacturonase and biomass production in submerged fermentation. Environmental Technology, 36(20), 2657-2667. doi:10.1080/09593330.2015.10429220959-3330http://doi.org/10.1080/09593330.2015.1042922http://hdl.handle.net/11147/5670The potential of important agro-industrial wastes, apple pomace (AP) and orange peel (OP) as C sources, was investigated in the maximization of polygalacturonase (PG), an industrially significant enzyme, using an industrially important microorganism Aspergillus sojae. Factors such as various hydrolysis forms of the C sources (hydrolysed-AP, non-hydrolysed-AP, hydrolysed-AP + OP, non-hydrolysed-AP + OP) and N sources (ammonium sulphate and urea), and incubation time (4, 6, and 8 days) were screened. It was observed that maximum PG activity was achieved at a combination of non-hydrolysed-AP + OP and ammonium sulphate with eight days of incubation. For the pre-optimization study, ammonium sulphate concentration and the mixing ratios of AP + OP at different total C concentrations (9, 15, 21 g-1) were evaluated. The optimum conditions for the maximum PG production (144.96 ml-1) was found as 21 g-1 total carbohydrate concentration totally coming from OP at 15 g-1 ammonium sulphate concentration. On the other hand, 3:1 mixing ratio of OP + AP at 11.50 g-1 ammonium sulphate concentration also resulted in a considerable PG activity (115.73 ml-1). These results demonstrated that AP can be evaluated as an additional C source to OP for PG production, which in turn both can be alternative solutions for the elimination of the waste accumulation in the food industry with economical returns.eninfo:eu-repo/semantics/openAccessApple pomaceAspergillus sojaeOrange peelPolygalacturonaseIndustrial wasteAmmonium sulfateEvaluation of Agro-Industrial Wastes, Their State, and Mixing Ratio for Maximum Polygalacturonase and Biomass Production in Submerged FermentationArticle2-s2.0-8493896241810.1080/09593330.2015.1042922