Özek, GülmiraBedir, ErdalTabanca, NurhayatAli, AbbasKhan, Ikhlas A.Duran, AhmetBaşer, Kemal H.C.Özek, Temel2018-11-262018-11-262018Özek, G. Bedir, E., Tabanca, N., Ali, A., Khan, I.A., Duran, A., Başer, K.H.C., and Özek, T. (2018). Isolation of eudesmane type sesquiterpene ketone from Prangos heyniae H.Duman & M.F.Watson essential oil and mosquitocidal activity of the essential oils. Open Chemistry, 16(1), 453-467. doi:10.1515/chem-2018-00512391-54202391-5420http://doi.org/10.1515/chem-2018-0051https://hdl.handle.net/11147/7017In the present work, an endemic species Prangos heyniae collected in four locations from Turkey was subjected to hydrodistillation in Clevenger type apparatus to obtain the essential oils (EO1-4). The gas-chromatography/mass spectrometry (GC/MS) and gas-chromatography-flame ionization detector (GC/FID) analyses showed that the EOs were rich in sesquiterpenes, germacrene D (10.3-12.1%), β-bisabolene (14.4%), kessane (26.9%), germacrene B (8.2%), elemol (3.4-46.9%), β-bisabolenal (1.4-70.7%), β-bisabolenol (8.4%) and an eudesmane type sesquiterpene (1) (16.1%) with [M+218]. This unidentified compound (1) was isolated in a rapid one-step manner with >95.0% purity using Preparative Capillary Gas Chromatography (PCGC) with an HP Innowax column connected to a Preparative Fraction Collector (PFC) system. Structure determination was accomplished from 1D- and 2D-NMR spectroscopic data which determined a new eudesmane type sesquiterpene, 3,7(11)-eudesmadien-2-one (1). Using a biting deterrent bioassay, the mean proportion not biting (PNB) values of the P. heyniae EO1-4 were 0.88 for EO1 and 0.80 for EO2 which were similar to the positive control DEET (N,N-diethyl-3-methylbenzamide). The EO3 and EO4 had lower PNB values of 0.64 and 0.44, respectively. P. heyniae EO1-4 showed good larvicidal activity at 125 and 62.5 ppm whereas EO1-3 were slightly less effective at the dose of 31.25 ppm and EO4 was not active at 31.25 ppm against 1st instar Aedes aegypti.enAttribution-NonCommercial-NoDerivs 3.0 United Statesinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/3.0/us/Aedes aegyptiPrangos heyniaeNMRLarvicidal and biting deterrentPreparative gas chromatographyArticle2-s2.0-8504780939210.1515/chem-2018-005110.1515/chem-2018-0051