İplik, Elif SinemERtuğrul, BarışKozanoğlu, İlknurBaran, Yusuf2020-01-082020-01-082018İplik, E. S., Ertuğrul, B., Kozanoğlu, İ., and Baran, Y. (2018). An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue. Iranian Journal of Basic Medical Sciences, 21(5), 465-468. doi:10.22038/IJBMS.2018.26152.642020083874200838662008-3866https://doi.org/10.22038/IJBMS.2018.26152.6420https://hdl.handle.net/11147/7569Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer. Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured. Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells. Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.eninfo:eu-repo/semantics/openAccessApoptosisCell deathColon cancerStem cellsArticle2-s2.0-8504529875810.22038/IJBMS.2018.26152.642010.22038/IJBMS.2018.26152.6420