Please use this identifier to cite or link to this item:
https://hdl.handle.net/11147/10162
Title: | Xenograft tumor volume measurement in nude mice: Estimation of 3D ultrasound volume measurements based on manual caliper measurements | Authors: | Barış, Mustafa Serinan, Efe Çalışır, Meryem Şimşek, Kürşat Aktaş, Safiye Yılmaz, Osman Seçil, Mustafa Kılıç Özdemir, Sevgi |
Publisher: | Dokuz Eylül Üniversitesi | Abstract: | Objectives: Volume measurement of subcutaneous xenograft tumors in nude mice models is an important metric to assess tumor growth or response to therapy. Manual calipers are widely used for this purpose. But the measurements with manual calipers may be inaccurate. Contrarily, three-dimensional (3D) ultrasonographic measurements give reliable and accurate tumor volume calculation. We aim to; evaluate the accuracy of common four formulas given in the literature to estimate xenograft tumor volumes based on manual caliper measurements and offer a new coefficient for a better estimation of the tumor volumes. Patients and Methods: Detailed manual diameter measurements of xenograft tumors were in 14 nude mice performed using Vernier caliper. Tumor volumes were calculated using the suggested formulas in the literature based on manual measurements. 3D ultrasound volume measurements were performed on same xenograft tumors using high resolution Vevo 2100 imaging system. To propose a new coefficient; means of ratio between manual and ultrasound volume measurement values were used. Also, data set was divided into two subgroups as tumor volume under 800 mm3 and over 800 mm3. New coefficients for each subgroup were defined. Results: Only with prolate ellipsoid formula there was no statistically significant difference between volume measurements with two methods (p=0,24). Our proposed formula (0,45 L*W*H) could estimate tumor volumes as good as prolate ellipsoid formula. Coefficient 0,35 and 0,81 in the same formula were found efficient in the selected subgroups. Conclusion: Using one common coefficient/formula for tumor volume estimation in any tumor size can be inaccurate. Appropriate coefficient should be chosen according to the dataset worked with. | URI: | https://doi.org/10.30621/jbachs.2020.902 https://hdl.handle.net/11147/10162 https://search.trdizin.gov.tr/yayin/detay/370778 |
ISSN: | 2458-8938 2564-7288 |
Appears in Collections: | Chemical Engineering / Kimya Mühendisliği TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
Files in This Item:
File | Size | Format | |
---|---|---|---|
10.30621-jbachs.2020.902-1460521.pdf | 2.37 MB | Adobe PDF | View/Open |
CORE Recommender
WEB OF SCIENCETM
Citations
6
checked on Aug 24, 2024
Page view(s)
9,936
checked on Sep 2, 2024
Download(s)
294
checked on Sep 2, 2024
Google ScholarTM
Check
Altmetric
Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.