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Title: Scaffold-free biofabrication of adipocyte structures with magnetic levitation
Authors: Sarıgil, Öykü
Anıl İnevi, Müge
Fıratlıgil Yıldırır, Burcu
Ünal, Yağmur Ceren
Yalçın Özuysal, Özden
Meşe, Gülistan
Özçivici, Engin
Izmir Institute of Technology
Izmir Institute of Technology
Izmir Institute of Technology
Izmir Institute of Technology
Izmir Institute of Technology
Izmir Institute of Technology
Izmir Institute of Technology
Keywords: Adipose tissue
Bone marrow stem cells
Magnetic levitation
Tissue engineering
Single cell studies
Issue Date: Mar-2021
Publisher: John Wiley and Sons Inc.
Abstract: Tissue engineering research aims to repair the form and/or function of impaired tissues. Tissue engineering studies mostly rely on scaffold-based techniques. However, these techniques have certain challenges, such as the selection of proper scaffold material, including mechanical properties, sterilization, and fabrication processes. As an alternative, we propose a novel scaffold-free adipose tissue biofabrication technique based on magnetic levitation. In this study, a label-free magnetic levitation technique was used to form three-dimensional (3D) scaffold-free adipocyte structures with various fabrication strategies in a microcapillary-based setup. Adipogenic-differentiated 7F2 cells and growth D1 ORL UVA stem cells were used as model cells. The morphological properties of the 3D structures of single and cocultured cells were analyzed. The developed procedure leads to the formation of different patterns of single and cocultured adipocytes without a scaffold. Our results indicated that adipocytes formed loose structures while growth cells were tightly packed during 3D culture in the magnetic levitation platform. This system has potential for ex vivo modeling of adipose tissue for drug testing and transplantation applications for cell therapy in soft tissue damage. Also, it will be possible to extend this technique to other cell and tissue types.
Description: PubMed: 33205833
ISSN: 0006-3592
Appears in Collections:Bioengineering / Biyomühendislik
Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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