Please use this identifier to cite or link to this item:
|Title:||Pseudomonas aeruginosa presents multiple vital changes in its proteome in the presence of 3-hydroxyphenylacetic acid, a promising antimicrobial agent||Authors:||Özdemir, Özgün O.
|Issue Date:||2020||Publisher:||American Chemical Society||Abstract:||Pseudomonas aeruginosa, a widely distributed opportunistic pathogen, is an important threat to human health for causing serious infections worldwide. Due to its antibiotic resistance and virulence factors, it is so difficult to combat this bacterium; thus, new antimicrobial agents are in search. 3-Hydroxyphenylacetic acid (3-HPAA), which is a phenolic acid mostly found in olive oil wastewater, can be a promising candidate with its dose-dependent antimicrobial properties. Elucidating the molecular mechanism of action is crucial for future examinations and the presentation of 3-HPAA as a new agent. In this study, the antimicrobial activity of 3-HPAA on P. aeruginosa and its action mechanism was investigated via shot-gun proteomics. The data, which are available via ProteomeXchange with identifier PXDO16243, were examined by STRING analysis to determine the interaction networks of proteins. KEGG Pathway enrichment analysis via the DAVID bioinformatics tool was also performed to investigate the metabolic pathways that undetected and newly detected groups of the proteins. The results displayed remarkable changes after 3-HPAA exposure in the protein profile of P. aeruginosa related to DNA replication and repair, RNA modifications, ribosomes and proteins, cell envelope, oxidative stress, as well as nutrient availability. 3-HPAA showed its antimicrobial action on P. aeruginosa by affecting multiple bacterial processes; hence, it could be categorized as a multitarget antimicrobial agent.||URI:||https://doi.org/10.1021/acsomega.0c00703
|Appears in Collections:||Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik|
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Show full item record
checked on Dec 2, 2023
WEB OF SCIENCETM
checked on Jun 17, 2023
checked on Dec 4, 2023
checked on Dec 4, 2023
Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.