Please use this identifier to cite or link to this item:
https://hdl.handle.net/11147/12662
Title: | Protocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells | Authors: | Ayaz Güner, Şerife Acar, Mustafa Burak Boyvat, Dudu Güner, Hüseyin Bozalan, Habibe Güzel, Melis Yıldır, Selin Kübra Altınsoy, Nilay Fındık, Fatma Karakükçü, Musa Özcan, Servet |
Keywords: | Cell biology Cell isolation Mass spectrometry Protein biochemistry Proteomics |
Publisher: | Elsevier | Abstract: | Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs). We describe the steps for isolating target cells and their in-column surface biotinylation, followed by isolation and mass spectrometry analysis of biotinylated proteins. The protocol enables in-column surface biotinylation of specific cell subsets with minimal membrane disruption. | URI: | https://doi.org/10.1016/j.xpro.2022.101863 https://hdl.handle.net/11147/12662 |
ISSN: | 2666-1667 |
Appears in Collections: | Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
1-s2.0-S2666166722007432-main.pdf | Article File | 5.53 MB | Adobe PDF | View/Open |
CORE Recommender
Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.