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Title: Protocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells
Authors: Ayaz Güner, Şerife
Acar, Mustafa Burak
Boyvat, Dudu
Güner, Hüseyin
Bozalan, Habibe
Güzel, Melis
Yıldır, Selin Kübra
Altınsoy, Nilay
Fındık, Fatma
Karakükçü, Musa
Özcan, Servet
Keywords: Cell biology
Cell isolation
Mass spectrometry
Protein biochemistry
Publisher: Elsevier
Abstract: Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs). We describe the steps for isolating target cells and their in-column surface biotinylation, followed by isolation and mass spectrometry analysis of biotinylated proteins. The protocol enables in-column surface biotinylation of specific cell subsets with minimal membrane disruption.
ISSN: 2666-1667
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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