Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/12840
Title: Identification of Staphylococcus aureus cheese isolates with respect to virulence properties, genetic relatedness and antibiotic resistance profiles
Authors: Kadiroğlu, Pınar
Korel, Figen
Ceylan, Çağatay
Keywords: Staphylococcus aureus
PCR
Identification
Antibiotic resistance
Issue Date: 2019
Publisher: Özkan Özden
Abstract: The problems on identification of Staphylococcus aureus isolates from cheese samples wereinvestigated by phenotypic and genotypic tests in this study. Among 207 Staphylococcus spp.isolated from 31 cheese samples, 23 isolates that were Gram positive, catalase and slide coagulasepositive, with 1 isolate that was latex agglutination test negative showed different phenotypicproperties. Polymerase chain reaction (PCR) and quantitative PCR (qPCR) analyses showed thatDNase test and target genes (nuc, coa) regarded as gold standard regions for S. aureus were notfound to be unique for identification of S. aureus. The toxin genes (SEA-SEE) were not detected byPCR. Antibiotic resistance profiles of S. aureus isolates demonstrated that two isolates were resistantto penicillin G. This study showed that the unique phenotypic and genotypic test was not adequatefor identification of S. aureus isolates. There was no correlation between the presence of the nucgene and toxin genes. The presence of nuc gene which was used for detection of S. aureus was alsofound to be present in other Staphylococcus isolates. As a conclusion, the results revealed thatbiochemical tests could lead to false positive results for identification of S. aureus. The presence ofnuc gene is not correlated with the presence of toxin genes.
URI: https://doi.org/10.3153/FH19016
https://hdl.handle.net/11147/12840
https://search.trdizin.gov.tr/yayin/detay/387996
Appears in Collections:Food Engineering / Gıda Mühendisliği
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
document.pdfArticle File531.78 kBAdobe PDFView/Open
Show full item record



CORE Recommender

Page view(s)

84
checked on Feb 26, 2024

Download(s)

16
checked on Feb 26, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.