Please use this identifier to cite or link to this item:
https://hdl.handle.net/11147/13819
Title: | Antiproliferative and apoptotic effects of olive leaf extract microcapsules on MCF-7 and A549 cancer cells | Authors: | Bal, Yıldız Sürmeli, Yusuf Şanlı Mohamed, Gülşah |
Keywords: | Olive leaf extract Alginate microcapsules Oleuropein |
Publisher: | American Chemical Society | Abstract: | Alginate microcapsules are a talented means for the delivery of broad curative biomacromolecules. In this study, we immobilized olive leaf extract (OLE) by calcium alginate (CA) and chitosan-coated CA (CCA) and characterized the OLE-loaded CA and CCA. The cytotoxic effect, the cell cycle arrest, and the apoptotic effect of OLE and its microcapsules were investigated against breast adenocarcinoma (MCF-7) and lung carcinoma (A549). As a result, the loading capacity of OLE-CA and OLE-CCA was found to be 80 and 99%, respectively, in optimal conditions. Also, OLE-CA and OLE-CCA were characterized by unique FTIR peaks and morphological display relative to the empty CCA microcapsules. The cytotoxicity analysis showed that the IC50 values of OLE-CA and OLE-CCA were determined to be 312 and 0.94 μg mL-1 against A549, respectively, whereas these were found to be 865.4 and 425.5 μg mL-1 for MCF-7 cells. On the other hand, the OLE microcapsules did not possess in any concentration of cytotoxic influence on the BEAS 2B healthy cell line. Also, the exposure of OLE-CCA to MCF-7 and A549 resulted in the arrest of more MCF-7 and A549 cells at the G0/G1 phase compared to the OLE. A549 and MCF-7 cells were predominantly found in the late apoptosis phase and necrosis phase, respectively. Optical microscopy images confirmed that OLE microcapsules were more effective against MCF-7 and A549 than free OLE. The present work suggested that the OLE microcapsules might be administered as nutrition supplements for cancer therapy. © 2023 The Authors. Published by American Chemical Society. | URI: | https://doi.org/10.1021/acsomega.3c01493 https://hdl.handle.net/11147/13819 |
ISSN: | 2470-1343 |
Appears in Collections: | Bioengineering / Biyomühendislik Chemistry / Kimya PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
Files in This Item:
File | Size | Format | |
---|---|---|---|
Antiproliferative.pdf | 7.76 MB | Adobe PDF | View/Open |
CORE Recommender
SCOPUSTM
Citations
1
checked on Mar 29, 2024
WEB OF SCIENCETM
Citations
2
checked on Mar 16, 2024
Page view(s)
46
checked on Apr 22, 2024
Download(s)
8
checked on Apr 22, 2024
Google ScholarTM
Check
Altmetric
Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.