Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/14379
Title: Exploring Neuronal Differentiation Profiles in Sh-Sy5y Cells Through Magnetic Levitation Analysis
Authors: Kartal, Rumeysa Bilginer
Yildiz, Ahu Arslan
Keywords: [No Keyword Available]
Publisher: Amer Chemical Soc
Abstract: Magnetic levitation (MagLev) is a powerful and versatile technique that can sort objects based on their density differences. This paper reports the sorting of SH-SY5Y cells for neuronal differentiation by the MagLev technique. Herein, SH-SY5Y cells were differentiated with retinoic acid (RA) and brain-derived neurotrophic factor (BDNF). Neuronal differentiation was confirmed by neurite extension measurement and the immunostaining assay. Neurites reached the maximum length on day 9 after sequential treatment with RA-BDNF. Neuronal marker expression of un-/differentiated cells was investigated by beta-III tubulin and neuronal nuclei (NeuN) and differentiated cells exhibited a higher fluorescence intensity compared to un-/differentiated cells. MagLev results revealed that the density of differentiated SH-SY5Y cells gradually increased from 1.04 to 1.06 g/mL, while it remained stable at 1.05 g/mL for un-/differentiated cells. These findings signified that cell density would be a potent indicator of neuronal differentiation. Overall, it was shown that MagLev methodology can provide rapid, label-free, and easy sorting to analyze the differentiation of cells at a single-cell level.
Description: Arslan Yildiz, Ahu/0000-0003-0348-0575
URI: https://doi.org/10.1021/acsomega.3c08962
https://hdl.handle.net/11147/14379
ISSN: 2470-1343
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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