Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/2672
Title: Thermal stability of carbonic anhydrase immobilized within polyurethane foam
Authors: Kanbar, Bora
Özdemir, Ekrem
Keywords: Carbonic anhydrase
CO2 sequestration
Thermal stability
Enzymes
Polyurethane foam
Immobilization
Issue Date: Sep-2010
Publisher: John Wiley and Sons Inc.
Source: Kanbar, B., and Özdemir, E. (2010). Thermal stability of carbonic anhydrase immobilized within polyurethane foam. Biotechnology Progress, 26(5), 1474-1480. doi:10.1002/btpr.452
Abstract: Thermal stability of carbonic anhydrase (CA) immobilized within polyurethane (PU) foam was investigated. The catalytic activity of the enzyme was estimated by using p-nitrophenyl acetate (p-NPA) as the substrate in tris buffer containing 10% acetonitrile. The immobilized CA was stable during the repeatable washings and stability tests over 45 days stored in tris buffer at ambient conditions indicating that the CA was covalently attached to the polyurethane (PU) foam by crosslinking. The immobilized CA was found to be 98% stable below 50°C, whereas a drastic decrease was seen at temperatures between 50 and 60°C. The optimum temperature for the immobilized CA was found to be 45°C and it lost its activity completely at 60°C. Thermal deactivation energies for the free and immobilized CA were estimated to be 29 and 86 kcal/mol, respectively. The association of unfolded CA with the polymeric backbone chains of the PU foam was also addressed. It was concluded that the immobilized CA was highly stable at temperatures less than 50°C and could be used in biomimetic CO sequestration processes. © 2010 American Institute of Chemical Engineers
URI: http://doi.org/10.1002/btpr.452
http://hdl.handle.net/11147/2672
ISSN: 1520-6033
1520-6033
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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