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Title: Commercially suitable pectin methylesterase from Valencia orange peels
Other Titles: Valencia portakal kabuklarından ticari olabilecek pektin metilesteraz eldesi
Authors: Şimşek, Şebnem
Yemenicioğlu, Ahmet
Keywords: Solvent extraction
Valencia orange
Orange peel
Pectin methylesterase
Issue Date: 2010
Publisher: Türkiye Klinikleri Journal of Medical Sciences
Source: Şimşek, Ş., and Yemenicioğlu, A. (2010). Commercially suitable pectin methylesterase from Valencia orange peels. Turkish Journal of Agriculture and Forestry, 34(2), 109-119. doi:10.3906/tar-0903-7
Abstract: A simple and effective procedure was developed to extract pectin methylesterase (PME) from Valencia orange peels. Orange peels contain 25-34 μmol of COOH min-1 g-1 of peel PME activity. The enzyme was ionically bound to cell walls and could not be extracted with water. This enables removal of water soluble pectic substances and oils from peels via homogenization and washing with water before enzyme extraction. Enzyme extraction can be conducted simply by addition of suitable amounts of NaCl (optimum: 10 g of NaCl 100 g-1 of extraction mixture) to peel homogenate and stirring (optimum: 30 min at 200 rpm). The PME extracted from orange peels contains almost the same amount of heat-stable and heat-labile fraction, and the enzymes cannot be activated by mild heating. A slight activation of enzyme (almost 20%) was achieved by adding 1 mM CaCl2 to enzyme extracts, but this agent was inhibitory at higher concentrations. The extracts stabilized by Na-benzoate and K-sorbate maintained more than 90% of their PME activity at 4 °C for at least 5 months. The obtained PME was successfully used to prepare low-methoxyl citrus pectin used in edible film formation in the presence of CaCl2. This study shows the potential of using Valencia orange peels as a source of commercial PME. © TÜBİTAK.
ISSN: 1300-011X
Appears in Collections:Food Engineering / Gıda Mühendisliği
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
TR Dizin İndeksli Yayınlar / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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