Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/4305
Title: Invadopodia formation on nanometer scale protein patterns
Other Titles: Nanometre ölçeğindeki protein desenleri üzerinde işgalci-ayak oluşumu
Authors: Pesen Okvur, Devrim
Özyüzer, Lütfi
Batı, Gizem
Batı, Gizem
Izmir Institute of Technology. Biotechnology and Bioengineering
Keywords: Biotechnology
Cancer cells
Issue Date: 2014
Publisher: Izmir Institute of Technology
Source: Batı, G. (2014). Invadopodia formation on nanometer scale protein patterns. Unpublished master's thesis, İzmir Institute of Technology, İzmir, Turkey
Abstract: How the positions of invadopodium in the cell are determined and if they have an adhesivefunction are not known. Using fluorescence microscopy and antibodies that recognize actin, cortactin and MT1-MMP proteins, invadopodia formed by breast cancer cells plated on protein nanopatterns of different geometeries and components after stimulation with epidermal growth factor which is known to induce invadopodia formation, were examined. Invadopodia formation was studied for the first time on nanometer scale, single and double active component, protein patterns with equal distance and gradient spacings. The results show that: • On K-casein-fibronectin nanopatterns, invadopodia prefer to form on K-casein which blocks cell adhesion rather than on fibronectin nanodots which promote cell adhesion. • On Laminin-fibronectin nanopatterns, invadopodia prefer to form on laminin rather than on fibronectin nanodots. • On gradient patterns, invadopodia prefer areas with wide spacings. These results support the hypotheses that the positions where invadopodia form can be determined by surface protein nanopatterns and that cell adhesion is not required at points where invadopodia will form.
Description: Thesis (Master)--Izmir Institute of Technology, Biotechnology and Bioengineering, Izmir, 2014
Full text release delayed at author's request until 2018.01.26
Includes bibliographical references (leaves: 104-109)
Text in English; Abstract: Turkish and English
xvi, 109 leaves
URI: http://hdl.handle.net/11147/4305
Appears in Collections:Master Degree / Yüksek Lisans Tezleri

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