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Title: Biophysical and microbiological study of high hydrostatic pressure inactivation of Bovine Viral Diarrheavirus type 1 on serum
Authors: Ceylan, Çağatay
Severcan, Feride
Özkul, Aykut
Severcan, Mete
Bozoğlu, Faruk
Taheri, Nusret
Keywords: Artificial neural networks
Fetal bovine serum
Fourier transform infrared spectroscopy
High hydrostatic pressure
Issue Date: Jan-2012
Publisher: Elsevier Ltd.
Source: Ceylan, Ç., Severcan, F., Özkul, A., Severcan, M., Bozoğlu, F., and Taheri, N. (2012). Biophysical and microbiological study of high hydrostatic pressure inactivation of Bovine Viral Diarrheavirus type 1 on serum. Veterinary Microbiology, 154(3-4), 266-271. doi:10.1016/j.vetmic.2011.07.026
Abstract: The effect of high hydrostatic pressure application on fetal bovine serum components and the model microorganism (Bovine Viral Diarrheavirus type 1 NADL strain) was studied at 132 and 220MPa pressure for 5min at 25°C. Protein secondary structures were found to be unaffected by an artificial neural network application on the amide I region for both untreated and HHP treated samples. FTIR spectroscopy study of both the HHP-treated and control samples revealed changes in the intensity of some bands in the finger-print region (1500-900cm -1) originating mainly from lipids which are thought to result from changes in the lipoprotein structure. The virus strain lost its infectivity completely after 220MPa HHP treatments. These results indicate that HHP can be successfully used for inactivation of pestiviruses while leaving structural and functional properties of serum and serum products unaffected. © 2011 Elsevier B.V.
ISSN: 0378-1135
Appears in Collections:Food Engineering / Gıda Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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