Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5083
Title: Conjugation of siRNA with comb-type PEG enhances serum stability and gene silencing efficiency
Authors: Gunasekaran, Karthikeyan
Nguyen, Thi H.
Maynard, Heather D.
Davis, Thomas P.
Bulmuş, Volga
Keywords: PEG(meth)acrylate
siRNA
Biological applications of polymers
Polymer conjugates
RAFT polymerization
Reversible addition fragmentation chain transfer polymerization
Issue Date: Apr-2011
Publisher: John Wiley and Sons Inc.
Source: Gunasekaran, K., Nguyen, T.H., Maynard, H.D., Davis, T.P., and Bulmuş, V. (2001). Conjugation of siRNA with comb-type PEG enhances serum stability and gene silencing efficiency. Macromolecular Rapid Communications, 32(8), 654-659. doi:10.1002/marc.201000804
Abstract: A thiol-modified siRNA targeting the enhanced green fluorescence protein (eGFP) gene was conjugated with RAFT-synthesized, pyridyl disulfide-functional poly(PEG methyl ether acrylate)s (p(PEGA)s). siRNA-p(PEGA) conjugates demonstrated significantly enhanced in vitro serum stability and nuclease resistance compared to the unmodified and thiol-modified siRNA. The complexes of siRNA-p(PEGA) conjugates with a fusogenic peptide, KALA ((+)/(-) = 2) inhibited the protein expression approximately 28-fold more than the KALA complex of the unmodified siRNA. The protein inhibition caused by siRNA-p(PEGA)-KALA complexes (56± 5%-58± 3% of the fluorescence expressed in non-treated cells) was comparable to the effect of the unmodified siRNA-lipofectamine complex (77± 7%).
URI: https://doi.org/10.1002/marc.201000804
http://hdl.handle.net/11147/5083
ISSN: 1022-1336
1022-1336
1521-3927
Appears in Collections:Chemical Engineering / Kimya Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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