Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5240
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dc.contributor.authorBaran, Yusuf-
dc.contributor.authorCeylan, Çağatay-
dc.contributor.authorCamgöz, Aylin-
dc.date.accessioned2017-04-06T07:44:25Z-
dc.date.available2017-04-06T07:44:25Z-
dc.date.issued2013-04-
dc.identifier.citationBaran, Y., Ceylan, Ç., and Camgöz, A. (2013). The roles of macromolecules in imatinib resistance of chronic myeloid leukemia cells by Fourier transform infrared spectroscopy. Biomedicine and Pharmacotherapy, 67(3), 221-227. doi:10.1016/j.biopha.2012.12.001en_US
dc.identifier.issn0753-3322-
dc.identifier.urihttps://doi.org/10.1016/j.biopha.2012.12.001-
dc.identifier.urihttp://hdl.handle.net/11147/5240-
dc.description.abstractImatinib is a first generation tyrosine kinase inhibitor, which is used for the treatment of chronic myeloid leukemia. However, resistance to imatinib is an important problem. Different mechanisms have been explained for imatinib resistance. In this study, we examined the roles of macromolecules in imatinib resistance in K562 cells at the molecular level using Fourier Transform Infrared (FT-IR) spectroscopy. An amount of 3μM imatinib resistant cells were generated by our group and named as K562/IMA-3 cells. Changes in macromolecules in parental and resistant cells were studied by FT-IR spectroscopy. Imatinib resistance caused changes, which indicated decreases in the level of glycogen and increases in the membrane order. The amount of unsaturated lipids increased in the imatinib resistant cells indicating lipid peroxidation. Imatinib resistance caused changes in the lipid/protein ratio. The relative protein content increased with respect to nucleic acids indicating higher transcription and protein expression and structural/organizational changes in the nucleus were evident as revealed by frequency changes in the nucleic acid bands. Changes in the amide bands revealed changes in the proteome of the resistant cells. Protein secondary structural changes indicated that the antiparallel beta sheet's structure increased, however the alpha helix structure, beta sheet structure, random coil structure and turns decreased in the resistant cells. These results indicate that the FT-IR technique provides a suitable method for analyzing drug resistance related structural changes in leukemia and other cancer types.en_US
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.relation.ispartofBiomedicine and Pharmacotherapyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChronic myeloid leukemiaen_US
dc.subjectFourier transform infrared spectroscopyen_US
dc.subjectImatiniben_US
dc.subjectMultidrug resistanceen_US
dc.titleThe roles of macromolecules in imatinib resistance of chronic myeloid leukemia cells by Fourier transform infrared spectroscopyen_US
dc.typeArticleen_US
dc.authoridTR119193en_US
dc.authoridTR45775en_US
dc.institutionauthorBaran, Yusuf-
dc.institutionauthorCeylan, Çağatay-
dc.institutionauthorCamgöz, Aylin-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume67en_US
dc.identifier.issue3en_US
dc.identifier.startpage221en_US
dc.identifier.endpage227en_US
dc.identifier.wosWOS:000317098900008en_US
dc.identifier.scopus2-s2.0-84875533151en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.biopha.2012.12.001-
dc.identifier.pmid23433849en_US
dc.relation.doi10.1016/j.biopha.2012.12.001en_US
dc.coverage.doi10.1016/j.biopha.2012.12.001en_US
dc.identifier.wosqualityQ3-
dc.identifier.scopusqualityQ2-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
crisitem.author.dept03.08. Department of Food Engineering-
Appears in Collections:Food Engineering / Gıda Mühendisliği
Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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