Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5524
Title: Improved viability of Lactobacillus acidophilus NRRL-B 4495 during freeze-drying in whey protein-pullulan microcapsules
Authors: Çabuk, Burcu
Tellioğlu Harsa, Şebnem
Keywords: Encapsulation
Probiotics
Whey protein
Cryoprotective agents
Lactobacillus acidophilus
Pullulan
Issue Date: Apr-2015
Publisher: Informa Healthcare
Source: Çabuk, B., and Tellioğlu Harsa, Ş. (2015). Improved viability of Lactobacillus acidophilus NRRL-B 4495 during freeze-drying in whey protein-pullulan microcapsules. Journal of Microencapsulation, 32(3), 300-307. doi:10.3109/02652048.2015.1017618
Abstract: In this research, pullulan was incorporated in protein-based encapsulation matrix in order to assess its cryoprotective effect on the viability of freeze-dried (FD) probiotic Lactobacillus acidophilus NRRL-B 4495. This study demonstrated that pullulan in encapsulation matrix resulted in a 90.4% survival rate as compared to 88.1% for whey protein (WPI) encapsulated cells. The protective effects of pullulan on the survival of FD-encapsulated cells in gastrointestinal conditions were compared. FD WPI-pullulan capsules retained higher survived cell numbers (7.10 log CFU/g) than those of FD WPI capsules (6.03 log CFU/g) after simulated gastric juice exposure. Additionally, use of pullulan resulted in an increased viability after bile exposure. FD-free bacteria exhibited 2.18 log CFU/g reduction, while FD WPI and FD WPI-pullulan encapsulated bacteria showed 0.95 and 0.49 log CFU/g reduction after 24 h exposure to bile solution, respectively. Morphology of the FD microcapsules was visualized by scanning electron microscopy. © 2015 Informa UK Ltd.
URI: http://doi.org/10.3109/02652048.2015.1017618
http://hdl.handle.net/11147/5524
ISSN: 0265-2048
0265-2048
Appears in Collections:Food Engineering / Gıda Mühendisliği
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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