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https://hdl.handle.net/11147/5669
Title: | Polythiophene derivative on quartz resonators for miRNA capture and assay | Authors: | Palaniappan, Al. Cheema, Jamal Ahmed Rajwar, Deepa Ammanath, Gopal Xiaohu, Liu Seng Koon, Lim Yi, Wang Yıldız, Ümit Hakan Liedberg, Bo |
Keywords: | MicroRNAs Polymers Polythiophene Thiophenes Quartz crystal |
Publisher: | Royal Society of Chemistry | Source: | Palaniappan, A., Cheema, J. A., Rajwar, D., Ammanath, G., Xiaohu, L., Seng Koon, L., Yi, W., Yıldız, Ü. H., and Liedberg, B. (2015). Polythiophene derivative on quartz resonators for miRNA capture and assay, Analyst, 140(23), 7912-7917. doi:10.1039/c5an01663k | Abstract: | A novel approach for miRNA assay using a cationic polythiophene derivative, poly[3-(3′-N,N,N-triethylamino-1′-propyloxy)-4-methyl-2,5-thiophene hydrobromide] (PT), immobilized on a quartz resonator is proposed. The cationic PT enables capturing of all RNA sequences in the sample matrix via electrostatic interactions, resulting in the formation of PT-RNA duplex structures on quartz resonators. Biotinylated peptide nucleic acid (b-PNA) sequences are subsequently utilized for the RNA assay, upon monitoring the PT-RNA-b-PNA triplex formation. Signal amplification is achieved by anchoring avidin coated nanoparticles to b-PNA in order to yield responses at clinically relevant concentration regimes. Unlike conventional nucleic acid assay methodologies that usually quantify a specific sequence of RNA, the proposed approach enables the assay of any RNA sequence in the sample matrix upon hybridization with a PNA sequence complementary to the RNA of interest. As an illustration, successful detection of mir21, (a miRNA sequence associated with lung cancer) is demonstrated with a limit of detection of 400 pM. Furthermore, precise quantification of mir21 in plasma samples is demonstrated without requiring PCR and sophisticated instrumentation. | URI: | http://doi.org/10.1039/c5an01663k http://hdl.handle.net/11147/5669 |
ISSN: | 0003-2654 1364-5528 |
Appears in Collections: | Chemistry / Kimya PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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