Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5758
Title: Investigation of the structure of alpha-lactalbumin protein nanotubes using optical spectroscopy
Authors: Tarhan, Özgür
Tarhan, Enver
Harsa, Şebnem
Keywords: FTIR
Microscopy
Protein nanotubes
Raman
α-lactalbumin
Bacillus licheniformis
Issue Date: Feb-2014
Publisher: Cambridge University Press
Source: Tarhan, O., Tarhan, E., and Harsa, Ş. (2014). Investigation of the structure of alpha-lactalbumin protein nanotubes using optical spectroscopy. Journal of Dairy Research, 81(1), 98-106. doi:10.1017/S0022029913000629
Abstract: Alpha-lactalbumin (α-la) is one of the major proteins in whey. When partially hydrolysed with Bacillus licheniformis protease, it produces nanotubular structures in the presence of calcium ions by a self-assembly process. This study presents investigation of α-la protein structure during hydrolysis and nanotube formation using optical spectroscopy. Before spectroscopic measurements, nanotubes were examined with microscopy. The observed α-la nanotubes (α-LaNTs) were in the form of regular hollo strands with a diameter of about 20 nm and the average length of 1 μm. Amide and backbone vibration bands of the Raman spectra displayed remarkable conformational changes in α and β domains in the protein structure during nanotube growth. This was confirmed by the Fourier-transform infrared (FTIR) spectroscopy data. Also, FTIR analysis revealed certain bands at calcium (Ca++) binding sites of COO- groups in hydrolysed protein. These sites might be critical in nanotube elongation.
URI: https://doi.org/10.1017/S0022029913000629
http://hdl.handle.net/11147/5758
ISSN: 0022-0299
0022-0299
Appears in Collections:Food Engineering / Gıda Mühendisliği
Physics / Fizik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

Files in This Item:
File Description SizeFormat 
5758.pdfMakale844.29 kBAdobe PDFThumbnail
View/Open
Show full item record



CORE Recommender

SCOPUSTM   
Citations

18
checked on Feb 16, 2024

WEB OF SCIENCETM
Citations

15
checked on Jan 20, 2024

Page view(s)

1,652
checked on Feb 23, 2024

Download(s)

426
checked on Feb 23, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.