Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5822
Full metadata record
DC FieldValueLanguage
dc.contributor.authorDemir, Ayşe Banu-
dc.contributor.authorKoç, Ahmet-
dc.date.accessioned2017-06-30T11:20:03Z-
dc.date.available2017-06-30T11:20:03Z-
dc.date.issued2015-12-
dc.identifier.citationDemir, A.B., and Koç, A. (2015). High-copy overexpression screening reveals PDR5 as the main doxorubicin resistance gene in yeast. PLoS ONE, 10-12. doi:10.1371/journal.pone.0145108en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttps:/doi.org/10.1371/journal.pone.0145108-
dc.identifier.urihttp://hdl.handle.net/11147/5822-
dc.description.abstractDoxorubicin is one of the most potent anticancer drugs used in the treatment of various cancer types. The efficacy of doxorubicin is influenced by the drug resistance mechanisms and its cytotoxicity. In this study, we performed a high-copy screening analysis to find genes that play a role in doxorubicin resistance and found several genes (CUE5, AKL1, CAN1, YHR177W and PDR5) that provide resistance. Among these genes, overexpression of PDR5 provided a remarkable resistance, and deletion of it significantly rendered the tolerance level for the drug. Q-PCR analyses suggested that transcriptional regulation of these genes was not dependent on doxorubicin treatment. Additionally, we profiled the global expression pattern of cells in response to doxorubicin treatment and highlighted the genes and pathways that are important in doxorubicin tolerance/toxicity. Our results suggest that many efflux pumps and DNA metabolism genes are upregulated by the drug and required for doxorubicin tolerance.en_US
dc.description.sponsorshipTUBITAK (114Z701)en_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/KBAG/114Z701en_US
dc.relation.ispartofPLoS ONEen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDoxorubicinen_US
dc.subjectGenomic DNAen_US
dc.subjectMessenger RNAen_US
dc.subjectPDR5 proteinen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.titleHigh-copy overexpression screening reveals PDR5 as the main doxorubicin resistance gene in yeasten_US
dc.typeArticleen_US
dc.institutionauthorDemir, Ayşe Banu-
dc.institutionauthorKoç, Ahmet-
dc.departmentİzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume10en_US
dc.identifier.issue12en_US
dc.identifier.wosWOS:000367092300036en_US
dc.identifier.scopus2-s2.0-84956921472en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1371/journal.pone.0145108-
dc.identifier.pmid26690737en_US
dc.relation.doi10.1371/journal.pone.0145108en_US
dc.coverage.doi10.1371/journal.pone.0145108en_US
local.message.claim2022-06-06T11:56:50.142+0300|||rp00417|||submit_approve|||dc_contributor_author|||None*
dc.identifier.wosqualityQ1-
dc.identifier.scopusqualityQ1-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.openairetypeArticle-
crisitem.author.dept04.03. Department of Molecular Biology and Genetics-
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
Files in This Item:
File Description SizeFormat 
5822.PDFMakale1.09 MBAdobe PDFThumbnail
View/Open
Show simple item record



CORE Recommender

SCOPUSTM   
Citations

9
checked on Feb 16, 2024

WEB OF SCIENCETM
Citations

9
checked on Feb 26, 2024

Page view(s)

484
checked on Feb 26, 2024

Download(s)

242
checked on Feb 26, 2024

Google ScholarTM

Check




Altmetric


Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.